Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

58.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.9K
CRISPR01:59

CRISPR

18.9K
18.9K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.6K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.6K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.5K
Homologous Recombination02:31

Homologous Recombination

65.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
65.3K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

12.2K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
12.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Individual activity of forest rodents correlates to pathogen communities.

Scientific reports·2026
Same author

CRISPR-Cas-based activation of PPARGC1A boosts endogenous mitochondria and enhances cardiac function after myocardial infarction.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Semaglutide and tirzepatide: Oral cavity effects of weight-loss therapies.

EXCLI journal·2026
Same author

Biomolecular condensation of human IDRs initiates endogenous transcription via intrachromosomal looping or high-density promoter localization.

Nucleic acids research·2025
Same author

Biomolecular condensation of human IDRs initiates endogenous transcription via intrachromosomal looping or high-density promoter localization.

Nucleic acids research·2025
Same author

Characterization of Rationally Designed CRISPR/Cas9-Based DNA Methyltransferases with Distinct Methyltransferase and Gene Silencing Activities in Human Cell Lines and Primary Human T Cells.

ACS synthetic biology·2025

Related Experiment Video

Updated: Mar 27, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.7K

CRISPR-based Transcriptional Regulation: Technologies, Applications, and Future Directions.

Mira A Srinivasa1, Mario Escobar1,2

  • 1Department of Bioengineering, Rice University; Houston, TX 77005, USA.

DNA
|March 25, 2026
PubMed
Summary

CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) offer precise gene control for treating genetic diseases. These technologies are advancing rapidly, showing promise in clinical trials for various conditions.

Keywords:
CRISPR-Cas SystemsDNAEpigeneticsGenetic Engineering

More Related Videos

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.9K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.5K

Related Experiment Videos

Last Updated: Mar 27, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
07:49

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

Published on: May 30, 2025

2.7K
Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
10:46

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Published on: June 2, 2018

9.9K
Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models
13:47

Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

Published on: March 29, 2019

10.5K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Gene Therapy

Background:

  • CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) are advanced tools for precise gene expression control.
  • These technologies utilize modified CRISPR-Cas systems for targeted gene upregulation (CRISPRa) or silencing (CRISPRi).

Purpose of the Study:

  • To review the advancements and therapeutic potential of CRISPRa and CRISPRi technologies.
  • To highlight their application in various disease models and ongoing clinical trials.

Main Methods:

  • Utilizing nuclease-inactive Cas proteins fused with transcriptional activators (CRISPRa) or repressor domains (CRISPRi).
  • Employing guide RNAs for specific targeting of genes for activation or interference.
  • Evaluating preclinical data and clinical trial outcomes for CRISPRa/i therapies.

Main Results:

  • CRISPRa/i have shown efficacy in preclinical models of metabolic, neurological, muscular, and oncological diseases.
  • CRISPRi therapies are in clinical trials for hepatitis B and muscular dystrophy, demonstrating positive safety and efficacy.
  • Ongoing innovations are improving the precision and safety of CRISPR-based gene regulation.

Conclusions:

  • CRISPRa and CRISPRi represent significant progress in gene and epigenetic therapy.
  • These technologies are expected to revolutionize the treatment of genetic and epigenetic disorders.
  • Continued optimization will accelerate clinical adoption and expand therapeutic applications.