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High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method
Published on: May 21, 2018
In-vitro determination of ciprofloxacin - metronidazole interaction by derivative spectrophotometry with greenness
1Department of Pharmaceutical Chemistry, Dow College of Pharmacy, Dow University of Health Sciences, Karachi, Pakistan.
Background:
The study of DDIs has evolved significantly over the years, early in-vitro detections could help identify potential interactions before clinical use, enhancing drug efficacy and patient safety.
Objectives:
This work therefore focused on finding in-vitro DDI (incompatibility) between the two commonly prescribed antibiotics Ciprofloxacin (CFX) and Metronidazole (MTZ) in neutral, acidic and basic mediums using derivative spectroscopy.
Methods:
The spectral measurements were performed on UV-Visible spectrophotometer at 0, 4 and 24 hours. Standard solutions (100ppm) and working solutions (3-11ppm) of both drugs were prepared in water, while mixture contained 5ppm of each drug. AGREE calculator software was used to figure out the environmental impact of the used spectrophotometric method. A zero-crossing method was employed for simultaneous quantification of CFX and MTZ in admixture.
Results:
It was observed that a mixture having 5ppm CFX and MTZ showed a recovery percentage of 87.704% ± 0.001041 and 78.226% ± 0.002379 respectively in neutral medium at 4 hours, along with AGREE score of 0.8. The proposed method was greener (eco-friendly).
Conclusion:
The findings concluded that although the two drugs were compatible in acidic and basic mediums, these might be incompatible in neutral environment upon simultaneous administration.
