Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

3.5K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
3.5K
UV–Vis Spectroscopy: Woodward–Fieser Rules01:29

UV–Vis Spectroscopy: Woodward–Fieser Rules

29.5K
UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the...
29.5K
Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

1.8K
A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
1.8K
IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

6.0K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
6.0K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

3.6K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
3.6K
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

5.4K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
5.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detecting Vibrational Energy Transfer into an Enzyme Active Site via a Transition State Analog.

The journal of physical chemistry letters·2026
Same author

Molecular Mechanism of the Oxidative Cleavage of Alkenes by Photoexcited Nitroarenes.

Journal of the American Chemical Society·2026
Same author

Optimization of vibrationally promoted electronic resonance (VIPER) excitation.

Physical chemistry chemical physics : PCCP·2025
Same author

The slow photo-induced CO<sub>2</sub> release of <i>N</i>-phthaloylglycine.

Chemical science·2024
Same author

Time-Resolved Ion Mobility Mass Spectrometry to Solve Conformational Changes in a Cryptochrome.

Journal of the American Chemical Society·2024
Same author

Switch the click: Ultrafast photochemistry of photoDIBO-OH tracked by time-resolved IR spectroscopy.

The Journal of chemical physics·2024

Related Experiment Video

Updated: Mar 27, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

8.0K

Two-dimensional vibrational-electronic spectroscopy with a white light probe.

H M A Masood1, H Brunst1, L Denninger1

  • 1Institute of Biophysics, Goethe University Frankfurt am Main, Frankfurt am Main 60438, Germany.

The Review of Scientific Instruments
|March 25, 2026
PubMed
Summary
This summary is machine-generated.

We developed a new Fourier transform 2D-VE spectroscopy method to directly measure vibrational-electronic couplings. This technique resolves IR-excited electronic spectral changes, enabling clear identification of specific molecules even in complex mixtures.

More Related Videos

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

9.3K
Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.7K

Related Experiment Videos

Last Updated: Mar 27, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

8.0K
Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

9.3K
Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems
09:57

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional π-conjugate Systems

Published on: February 10, 2020

7.7K

Area of Science:

  • Spectroscopy
  • Chemical Physics
  • Physical Chemistry

Background:

  • Two-dimensional Vibrational-Electronic (2D-VE) spectroscopy is crucial for understanding vibrational and electronic transition couplings.
  • Existing methods often struggle with congested spectra and direct measurement of specific molecular interactions.

Purpose of the Study:

  • To present a novel Fourier transform 2D-VE spectroscopy setup.
  • To demonstrate its capability in resolving infrared (IR)-excited electronic spectral changes.
  • To showcase its application in disentangling complex molecular mixtures.

Main Methods:

  • Utilized a Fourier transform 2D-VE spectroscopy setup.
  • Employed compressed broadband white-light probing for spectral analysis.
  • Applied infrared (IR) excitation to induce electronic spectral modulations.

Main Results:

  • Successfully resolved IR-induced changes in electronic spectra.
  • Demonstrated mode- and molecule-specific vibronic couplings.
  • Distinguished specific laser dyes within a mixture with overlapping IR spectra.
  • Achieved measurements across a broad wavelength range (380–700 nm).

Conclusions:

  • The developed 2D-VE setup directly measures vibronic couplings.
  • This technique offers a powerful tool for analyzing complex molecular systems.
  • It provides a direct method for characterizing IR-induced electronic spectral changes.