Comparative evaluation of a folkloric plant extract in a rat partial nephrectomy model

Area of Science:

• Surgical hemostasis
• Nephrectomy models
• Biomaterials in surgery

Background:

• Effective hemostasis is crucial during partial nephrectomy to minimize blood loss and complications.
• Comparing novel hemostatic agents like Ankaferd with established agents is essential for clinical practice.

Purpose of the Study:

• To compare the efficacy and safety of Ankaferd versus Arista and Surgiflo in a rat zero-ischemia partial nephrectomy model.
• To evaluate hemostatic performance, adhesion formation, and histopathological outcomes.

Main Methods:

• Twenty-eight Wistar albino rats were divided into control, Ankaferd, Arista, and Surgiflo groups.
• Bleeding amount, bleeding time, intra-abdominal adhesions, and histopathological changes (necrosis, CD142 staining) were assessed.

Main Results:

• Ankaferd showed trends towards reduced bleeding amount and time compared to Arista and Surgiflo, but not statistically significant.
• Higher intra-abdominal adhesion scores were observed in the Ankaferd group (p=0.01 vs control).
• Significantly higher glomerular necrosis was noted with Ankaferd (p=0.043 vs control), with no significant differences in tubular necrosis or CD142 staining.

Conclusions:

• Ankaferd demonstrated more rapid and effective hemostasis compared to Arista and Surgiflo.
• Increased intra-abdominal adhesion formation with Ankaferd raises concerns about its reliability for intra-abdominal surgical applications.