Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modified-Release Drug Delivery Systems: Site-Targeted01:24

Modified-Release Drug Delivery Systems: Site-Targeted

94
Site-targeted drug delivery systems enhance therapeutic efficacy while minimizing systemic toxicity and treatment costs. Unlike conventional methods, these systems ensure precise drug delivery, improving bioavailability and reducing side effects. Targeted drug delivery is classified into three levels. First-order targeting directs drugs to the capillary beds of specific organs or tissues. Second-order targets specific cell types, such as tumor cells, using receptor-mediated interactions.
94
Site-Targeted Drug Delivery Systems: Polymeric Carriers01:24

Site-Targeted Drug Delivery Systems: Polymeric Carriers

108
Polymeric carriers enhance targeted drug delivery by increasing efficacy while minimizing off-target effects. These carriers comprise a biodegradable polymeric backbone integrated with functional elements that enable targeting, improve physicochemical properties, and regulate drug release.Targeting MechanismsThe targeting ability of polymeric carriers is mediated by a homing device, which is a molecular recognition component designed to selectively bind to specific tissues or cells. Monoclonal...
108

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasmonic nanocomposite helices for weather-adaptive LiDAR function.

Nature communications·2026
Same author

The Use of Deep Learning in RNA Therapeutic Development.

ACS nano·2026
Same author

A Water Extract of Mixed Mushroom Mycelia Mitigates Cognitive Deficit and Oxidative Stress After Global Cerebral Ischemia-Reperfusion Injury.

Current issues in molecular biology·2026
Same author

Interpretable Deep Learning for Single-Molecule Nanopore Fingerprinting Using Physics-Guided Preprocessing.

ACS sensors·2026
Same author

Enabling global-scale nucleic acid repositories through versatile, scalable biochemical selection from room-temperature archives.

Nature communications·2026
Same author

DNA origami vaccines program antigen-focused germinal centers.

Science (New York, N.Y.)·2026
Same journal

Tuning Piezoelectricity and Pyroelectricity in Poly(vinylidene fluoride) through Ionic Liquid Anion-Size Directed Polymorph and Interface Engineering.

ACS applied materials & interfaces·2026
Same journal

Adsorption-Induced Ferroelectric Symmetry Breaking in Two-Dimensional CuInP<sub>2</sub>S<sub>6</sub>.

ACS applied materials & interfaces·2026
Same journal

Nanocomplexes Integrated into a Polymeric Bilayer Film Enhance Buccal Permeation of a GLP-1 Peptide Analogue.

ACS applied materials & interfaces·2026
Same journal

Correction to "Multienzyme Active Nanozyme for Efficient Sepsis Therapy through Modulating Immune and Inflammation Inhibition".

ACS applied materials & interfaces·2026
Same journal

A Programmable Perfusion Platform with Temperature Monitoring Achieves Multiscale Cryopreservation.

ACS applied materials & interfaces·2026
Same journal

Oral Delivery of Mesenchymal Stem Cell-Derived Extracellular Vesicles To Treat Intestinal Inflammation.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: Mar 27, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

25.8K

Programmable Lipid Functionalization of Nucleic Acid Nanoparticles Modulates Liver Cell-Type Targeting.

Hyun Min Kim1, Marjan Omer1,2, Grant A Knappe1,3

  • 1Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, United States.

ACS Applied Materials & Interfaces
|March 25, 2026
PubMed
Summary
This summary is machine-generated.

Nucleic acid nanoparticles (NANPs) show promise for delivering nucleic acid therapeutics. This study reveals how lipid modifications enhance NANP uptake in liver cells by optimizing receptor engagement and endocytosis for improved therapeutic delivery.

Keywords:
DNA origamibiomolecular coronadrug deliverymultivalencynanoparticlesnucleic acid therapeutics

More Related Videos

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries
08:10

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Published on: August 23, 2024

6.6K
Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells
10:02

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

2.8K

Related Experiment Videos

Last Updated: Mar 27, 2026

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform
09:41

Formulating and Characterizing Lipid Nanoparticles for Gene Delivery using a Microfluidic Mixing Platform

Published on: February 25, 2021

25.8K
Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries
08:10

Author Spotlight: Enhancing Lipid Nanoparticle Formation Through Turbulent Mixing in Confined Geometries

Published on: August 23, 2024

6.6K
Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells
10:02

Using Lipid Nanoparticles for the Delivery of Chemically Modified mRNA into Mammalian Cells

Published on: June 10, 2022

2.8K

Area of Science:

  • Biotechnology and Nanomedicine
  • Molecular Biology and Genetics
  • Drug Delivery Systems

Background:

  • Nucleic acid nanoparticles (NANPs) are promising vectors for nucleic acid therapeutics, offering precise ligand presentation.
  • Understanding the cellular targeting and uptake mechanisms of NANPs is crucial for optimizing their therapeutic potential.
  • Current knowledge on how NANPs interact with cells and deliver cargo remains limited.

Purpose of the Study:

  • To investigate the cellular targeting, uptake, and small interfering RNA (siRNA) delivery mechanisms of NANPs in liver and neuronal cell models.
  • To evaluate the impact of ligand design (valency, spacing, linker length, chemistry) on NANP interactions with liver cells and their receptors (ASGPR, LDLR).
  • To explore lipid functionalization as a strategy for targeting non-hepatic cells, specifically neuronal models.

Main Methods:

  • Fabrication of NANPs using DNA origami with targeted ligands (GalNAc for ASGPR, lipids for LDLR).
  • Systematic evaluation of ligand parameters (valency, spacing, linker length, lipophilicity) on NANP association with liver cell models (HepG2).
  • Assessment of NANP uptake pathways (clathrin-mediated endocytosis) and biomolecular corona formation.
  • Benchmarking NANP siRNA delivery efficiency against lipid nanoparticles and conjugate technologies.

Main Results:

  • Lipidation significantly enhanced NANP uptake in HepG2 cells by promoting apolipoprotein recruitment, LDLR engagement, and clathrin-mediated endocytosis.
  • Higher lipid valency improved HepG2 uptake when lipids were adequately spaced from the NANP surface; more lipophilic lipids increased cell association.
  • NANP lipidation also increased association with non-parenchymal liver cells, indicating potential off-target effects.
  • NANPs demonstrated potential for siRNA delivery to HepG2 cells and exploration of lipid functionalization for neuronal targeting.

Conclusions:

  • Ligand design, particularly lipidation and valency, critically influences NANP targeting and uptake in liver cells via specific receptor-mediated endocytosis.
  • Lipid functionalization is a viable strategy for enhancing NANP delivery to hepatocytes and potentially targeting non-hepatic cells like neurons.
  • This research provides foundational insights into NANP-ligand interactions, informing the design criteria for effective nucleic acid therapeutic delivery systems.