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Updated: Mar 27, 2026
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
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Proteomics in biopharmaceutical industries.
1Center for Genetic Engineering and Biotechnology, PO Box 6162, La Habana, Cuba.
Journal of Proteomics
|March 25, 2026
Summary
Proteomics and mass spectrometry are vital for biopharmaceutical drug discovery and production. The Multi-Attribute Method offers advanced quality control for therapeutic proteins, promising enhanced safety and efficiency.
Area of Science:
- Biopharmaceutical Science
- Analytical Chemistry
- Proteomics
Background:
- Proteomic tools have been essential in the biopharmaceutical industry for drug discovery and recombinant protein production.
- Liquid chromatography-mass spectrometry is a key analytical method for characterizing therapeutic proteins.
Purpose of the Study:
- To highlight the significance of proteomics in biopharmaceutical development and production.
- To introduce the Multi-Attribute Method (MAM) as an advanced analytical approach for quality control of therapeutic proteins.
Main Methods:
- Review of proteomic tools, including liquid chromatography-mass spectrometry.
- Focus on the Multi-Attribute Method for analyzing intact proteins.
Main Results:
- The Multi-Attribute Method is increasingly adopted for quality control in the biopharmaceutical industry.
- MAM enables simultaneous monitoring and quantification of multiple quality attributes in therapeutic proteins like monoclonal antibodies.
Conclusions:
- Proteomics plays a crucial role in biopharmaceutical innovation and manufacturing.
- Advances in mass spectrometry and liquid chromatography, particularly MAM, are poised to become fundamental for ensuring therapeutic protein quality and safety.
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