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Updated: Mar 27, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Systematic Analysis Reveals Two Novel O-Methyltransferase Genes Involved in Flavonoid Biosynthesis in Ginkgo Leaves
Manman Shi1,2, Xiaoming Yang1, Guibin Wang1
1State Key Laboratory for Development and Utilization of Forest Food Resources, Co-innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Abstract:
Flavonoids are widely distributed in plants, including Ginkgo biloba, a traditional medicinal and edible species. Methylated flavonoids often exhibit enhanced biological activities compared with their unmethylated forms, and flavonoid O-methyltransferases (FOMTs) responsible for this modification have been identified in several plants but remain poorly characterized in ginkgo. In this study, transcriptomic analysis of ginkgo leaves at different developmental stages identified two S-adenosylmethionine-dependent FOMTs, GbFOMT3 and GbFOMT12. Enzymatic assays with seven flavonoid substrates showed that both enzymes methylate quercetin, eriodictyol, and luteolin to produce isorhamnetin, hesperetin, and chrysoeriol, respectively, through methylation at the 3'-OH and 4'-OH positions of the B ring. These findings expand the repertoire of ginkgo flavonoid O-methyltransferases and provide insights into flavonoid methylation in ginkgo.
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