Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Systems-Based Practice Core Competency: A Survey of Physicians' Perspectives.

Cureus·2026
Same author

Gene expression dynamics in wound healing: Comparative analysis between the wound edge and center.

PloS one·2026
Same author

Towards adaptive bioelectronic wound therapy with integrated real-time diagnostics and machine learning-driven closed-loop control.

npj biomedical innovations·2026
Same author

A multi therapy bioelectronic wound dressing.

npj biomedical innovations·2026
Same author

Correction: A data-driven approach to establishing cell motility patterns as predictors of macrophage subtypes and their relation to cell morphology.

PloS one·2026
Same author

Characterization of cutaneous wound healing in swine.

JID innovations : skin science from molecules to population health·2026

Related Experiment Video

Updated: Mar 27, 2026

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells
08:34

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Published on: September 28, 2022

6.2K

A Flow Cytometry Workflow for Identifying Myeloid Subsets in Porcine Wound Tissue.

Shannon M Clayton1,2, Craig Collins1, Hsin-Ya Yang1

  • 1Department of Dermatology, School of Medicine, University of California, Davis, USA.

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [And] the European Tissue Repair Society
|March 26, 2026
PubMed
Summary

This study presents a new method for isolating porcine skin myeloid cells for wound healing research. This protocol aids in analyzing inflammatory and reparative processes in large animal models.

Keywords:
flow cytometrymyeloid cellsporcinewound healing

More Related Videos

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
07:21

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Published on: July 29, 2018

9.6K
Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
08:31

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

Published on: November 4, 2022

4.0K

Related Experiment Videos

Last Updated: Mar 27, 2026

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells
08:34

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Published on: September 28, 2022

6.2K
Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow
07:21

Identification and Isolation of Oligopotent and Lineage-committed Myeloid Progenitors from Mouse Bone Marrow

Published on: July 29, 2018

9.6K
Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry
08:31

Identification and Isolation of Burst-Forming Unit and Colony-Forming Unit Erythroid Progenitors from Mouse Tissue by Flow Cytometry

Published on: November 4, 2022

4.0K

Area of Science:

  • Veterinary Medicine
  • Immunology
  • Regenerative Medicine

Background:

  • Myeloid cells, including macrophages and granulocytes, are crucial for wound healing stages.
  • Pigs are a valuable large-animal model for studying skin injury and repair.
  • Existing methods for isolating porcine skin myeloid cells are insufficient for detailed analysis.

Purpose of the Study:

  • To develop and validate a protocol for isolating viable single myeloid cells from porcine skin wounds.
  • To enable detailed analysis of myeloid cell populations during wound healing in pigs.
  • To support future research on cellular mechanisms in porcine wound repair.

Main Methods:

  • Enzymatic digestion of porcine skin tissue post-excision injury.
  • Single-cell isolation ensuring high cell viability.
  • Application of isolated cells in flow cytometry and other downstream analyses.

Main Results:

  • Successful isolation of single myeloid cells from porcine skin wounds with high viability.
  • Demonstrated suitability of isolated cells for flow cytometry.
  • Characterization of myeloid cell populations across different wound healing stages.

Conclusions:

  • The presented enzymatic digestion protocol effectively isolates viable myeloid cells from porcine skin wounds.
  • This method facilitates the study of myeloid cell dynamics in porcine wound healing.
  • The protocol is valuable for future research into inflammation and repair mechanisms in this translational model.