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Related Experiment Video

Updated: Mar 27, 2026

Author Spotlight: Investigating the Impact of Nutrition on Mouse Brain Function and Metabolic Disorders

Author Spotlight: Investigating the Impact of Nutrition on Mouse Brain Function and Metabolic Disorders

Published on: September 6, 2024

A transcriptomic microglia taxonomy across mouse and human pathologies.

Chintan Chhatbar¹, Roman Sankowski^1,2, Michael Schulz¹

¹Institute of Neuropathology, Medical Faculty, University of Freiburg, Freiburg, Germany.

Nature Immunology

|March 26, 2026

Summary

This summary is machine-generated.

Researchers created a comprehensive atlas of over one million central nervous system cells, revealing a conserved taxonomy of myeloid cell states across diverse brain conditions. This framework aids in understanding immune cell roles in health and disease.

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Last Updated: Mar 27, 2026

Author Spotlight: Investigating the Impact of Nutrition on Mouse Brain Function and Metabolic Disorders

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Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Characterization and Isolation of Mouse Primary Microglia by Density Gradient Centrifugation

Published on: February 16, 2018

Area of Science:

Neuroscience
Immunology
Genomics

Background:

Single-cell studies highlight significant microglial diversity in brain development, homeostasis, and disease.
A unified framework is essential for comparing and stratifying microglial states across various contexts.

Purpose of the Study:

To generate a comprehensive atlas of myeloid cell states in the central nervous system (CNS).
To establish a taxonomy for stratifying microglial states across physiological and pathological conditions.
To map the spatial organization and interactions of CNS immune cells.

Main Methods:

Single-cell RNA sequencing of over one million CNS cells from more than 30 conditions.
Spatial transcriptomics to map immune cell states within tissue microenvironments.
In vivo perturbations to investigate signaling pathways regulating microglial states.

Main Results:

Developed a taxonomy of 27 superclusters and 192 clusters of myeloid cell states, largely conserved across diseases.
Mapped the spatial distribution and local interactions of immune cell states using spatial transcriptomics.
Demonstrated that activation-associated microglial states depend on interferon and CSF1R signaling.

Conclusions:

The study provides a spatially aware taxonomic framework for CNS immune cells in health and disease.
This atlas enables a deeper understanding of microglial diversity and function across the lifespan and in various pathologies.
The findings offer a foundation for future research into targeted immunotherapies for neurological disorders.