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The diagnostic performance of point-of-care tests for serum amyloid A and C-reactive protein in lame sows
Nadia Jakobsen1, Inge Larsen2, Nicolai Rosager Weber3
1Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark. najak@sund.ku.dk.
Background:
For acute phase proteins to be implemented in routine diagnostics for pig herds, a fast, reliable, and portable point-of-care test is required. Therefore, this study aimed to evaluate the diagnostic performance of two point-of-care tests on lame gestating sows. The two tests were the Cobas b101 CRP test for C-reactive protein and OmniChekTM-SAA for serum amyloid A. The reference test was an enzyme-linked immunosorbent assay (ELISA).
Results:
The C-reactive protein point-of-care test had a concordance with the ELISA results of 0.12 (95% confidence limits: 0.12-0.23) and a mean difference of - 30.98 µg/mL (Limits of agreement: 27.45 and - 89.40 µg/mL), with a clear proportional bias. The serum amyloid A point-of-care test was only in agreement with the ELISA results in two out of 49 samples. The serum amyloid A test overestimated the serum amyloid A levels, whereas the C-reactive protein point-of-care test underestimated the C-reactive protein levels.
Conclusions:
Currently, the two point-of-care tests cannot be reliably used to determine acute phase protein levels in blood samples from lame gestating sows.
