Ent-Labdane Diterpenoids from the Medicinal Plant Leucosceptrum canum

Area of Science:

• Natural Product Chemistry
• Pharmacology
• Medicinal Plant Research

Background:

• Leucosceptrum canum is a Lamiaceae medicinal plant with potential therapeutic compounds.
• Diterpenoids are a class of natural products known for diverse biological activities.

Purpose of the Study:

• To isolate and characterize new ent-labdane diterpenoids from Leucosceptrum canum.
• To evaluate the anti-inflammatory and anti-adipogenesis potential of the isolated compounds.

Main Methods:

• Isolation of compounds using chromatographic techniques.
• Structure elucidation via 1D/2D NMR, HRMS, and single-crystal X-ray diffraction.
• Biological assays including cytokine inhibition in LPS-induced macrophages and triglyceride accumulation in 3T3-L1 cells.

Main Results:

• Three new ent-labdane diterpenoids, leucolactones L-N (1-3), and one known analog (4) were identified.
• Compounds 1-3 demonstrated moderate anti-inflammatory effects, inhibiting TNF-α and IL-6 secretion (IC50: 15.43–25.88 µM).
• Compounds 1-3 also showed moderate anti-adipogenesis activity, inhibiting triglyceride accumulation (IC50: 12.20–22.55 µM).

Conclusions:

• Leucosceptrum canum is a source of novel ent-labdane diterpenoids with dual biological activities.
• The isolated compounds possess potential for development as anti-inflammatory and anti-obesity agents.