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Transdermal Measurement of Glomerular Filtration Rate in Mechanically Ventilated Piglets
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Feed restriction minimally affects nursery pig colonic function.

Sarah C Pearce1, Brian J Kerr1

  • 1National Laboratory for Agriculture and the Environment, USDA-ARS, Ames, IA 50011, United States.

Journal of Animal Science
|March 26, 2026
PubMed
Summary
This summary is machine-generated.

Feed restriction in pigs minimally impacts intestinal barrier function markers. Reduced crypt depth and altered gene expression (TLR2, LYZ) were observed, but key integrity markers remained unchanged.

Keywords:
feed restrictionintestinal permeability

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Area of Science:

  • Animal Science
  • Gastroenterology
  • Nutritional Physiology

Background:

  • Reduced feed intake is common post-weaning, during disease, or heat stress, leading to intestinal barrier dysfunction.
  • Current methods for assessing intestinal barrier function lack thorough validation.
  • Feed restriction offers a potential model to validate and compare intestinal integrity markers.

Purpose of the Study:

  • To investigate the impact of feed restriction on intestinal barrier function in nursery pigs.
  • To evaluate various in vivo, ex vivo, and tissue markers of intestinal integrity under controlled feed intake levels.
  • To assess colonic morphology, gene expression, and barrier function assays in response to feed restriction.

Main Methods:

  • Forty-eight barrows were fed at 100%, 75%, 50%, or 25% of ad libitum intake for 7 days.
  • Colon tissues were analyzed for ex vivo transepithelial electrical resistance (TEER) and fluorescein isothiocyanate-dextran (FD4) transport.
  • Colonic morphology, gene expression (CLDN4, TLR2, LYZ), and other barrier-related genes were examined.

Main Results:

  • No significant differences in TEER or FD4 transport were observed across feeding levels.
  • A significant decrease in colonic crypt depth was noted in pigs fed at 25% and 50% of intake (P < 0.05).
  • Gene expression of TLR2 increased significantly, while CLDN4 and LYZ showed trends towards altered expression in restricted groups.

Conclusions:

  • Feed restriction in nursery pigs resulted in minimal changes to key intestinal barrier function markers like TEER and FD4 transport.
  • Reduced crypt depth and altered immune-related gene expression (TLR2) suggest subtle impacts on colonic integrity.
  • Further research is needed to fully validate markers and understand the long-term consequences of feed restriction on intestinal health.