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Updated: Mar 27, 2026
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
Published on: January 21, 2020
Traumatic Occlusion Exacerbates Bone Resorption by Modifying Gene Expression in the Bone Tissue of Ligature-Induced
Yosuke Tsuchiya1, Yujin Ohsugi2,3,4, Tomomitsu Hirota5
1Department of Periodontology, Graduate School of Medical and Dental Sciences, Institute of Science Tokyo, Tokyo, Japan.
Aim:
To clarify the molecular mechanisms of occlusal trauma in bone loss through periodontal tissue transcriptome analysis in mice with periodontitis and traumatic occlusion.
Materials And Methods:
Ligature-induced periodontitis (Li) and composite resin-induced traumatic occlusion (Tra) mouse models were established (control [Co], Li, Tra and LiTra). Bone resorption was evaluated using micro-computed tomography (micro-CT). RNA-sequencing was conducted on gingiva, bone and periodontal ligament from all groups 3 days post induction. For long-term evaluation, the Co and Tra groups were maintained for 8 weeks and then analysed using micro-CT and qRT-PCR.
Results:
Traumatic occlusion alone, sustained for 8 weeks, did not directly induce bone resorption; however, it significantly exacerbated bone resorption in mice with periodontitis. Cytokine-cytokine receptor interactions and Toll-like receptor signalling pathways were up-regulated in LiTra bone tissue. Il11, Il1rl1 and Mmp3, associated with inflammation and bone metabolism, were more highly expressed in the LiTra group than in the Li group. TNF-α signalling via NFκB and inflammatory response gene sets were enriched in the bone tissue of LiTra group.
Conclusions:
Traumatic occlusion accelerates bone resorption in ligature-induced periodontitis but does not independently cause significant bone loss. Occlusal trauma enhances the expression of inflammation-related genes, especially in bone with periodontitis.
