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Published on: April 19, 2013
Gene polymorphisms and occurrence of type 2 diabetes in a Gabonese population
Aude S Mbang Bengone1, Rosalie Nikiema-Ndong1, Alvine S Batou1
1Department of Chemistry-Biochemistry, Faculty of Medicine, University of Health Science Libreville BP 4009, Libreville, Gabon.
Background:
Type 2 diabetes (T2DM) is a metabolic disease characterized by chronic hyperglycemia. We investigated the relationship between the polymorphisms rs5219 of KCNJ11, rs7903146 of TCF7L2, and rs13266634 of SLC30A8 in a Gabonese population with T2DM.
Method:
This case-control study was conducted at the Endocrinology Department of the University Hospital of Libreville. We enrolled 97 patients with T2DM and, from the general population, 87 control subjects who met the inclusion criteria. Genomic DNA was extracted with the Qiagen kit, and polymorphisms were analyzed using the RFLP method.
Results:
For KCNJ11, the wild type (EE) genotype frequency was 62.2% in controls versus 42.6% in cases, whereas the mutant type (KK) genotype frequency was 28.7% in cases versus 21.1% in controls (P=0.0243). For TCF7L2, the wild type (CC) genotype was found in 51.7% of controls and 37.1% of cases, and the mutant type (TT) genotype was found in 22.7% of cases versus 9.2% of controls (P=0.0253). For SLC30A8, the wild type (CC) genotype occurred in 26.0% of cases and 24.6% of controls (P=0.9326). After adjustment for age and body mass index, the recessive transmission models of rs5219 (KCNJ11) and rs7903146 (TCF7L2) were significantly associated with T2DM (P=0.021 and P=0.026, respectively).
Conclusion:
The rs5219 variant of KCNJ11 and the rs7903146 variant of TCF7L2 were associated with the occurrence of T2DM in this population.
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