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Updated: Mar 27, 2026

Lung microRNA Profiling Across the Estrous Cycle in Ozone-exposed Mice
Published on: January 7, 2019
Integrative linkage mapping and transcriptomic profiling uncover ozone-response modules in a peri-urban forest tree
Xochitl Granados-Aguilar1,2, Verónica Reyes-Galindo1,3, Gustavo I Giles-Pérez1
1Departamento de Ecología Evolutiva, Instituto de Ecología, Universidad Nacional Autónoma de México, Mexico City, CDMX 04510, Mexico.
Scientists mapped genes in Abies religiosa to understand ozone tolerance in Mexican forests. This research identifies key genes and pathways for managing conifer resistance to air pollution.
Area of Science:
- Genomics
- Plant Science
- Ecology
Background:
- Abies religiosa forests in Mexico are vital for ecosystem services but threatened by ozone pollution.
- Ozone exposure causes premature senescence and decline in these important peri-urban forests.
Purpose of the Study:
- To construct a saturated linkage map for Abies religiosa.
- To identify genes associated with ozone tolerance and understand their regulation.
Main Methods:
- Genotyping of 182 megagametophytes for 9,702 single nucleotide polymorphisms (SNPs).
- Construction of a 12-linkage group (LG) map spanning 1,567.88 cM.
- Differential gene expression and co-expression analyses in trees exposed to varying ozone levels.
Main Results:
- A saturated linkage map with 12 LGs was generated, locating 5,881 coding genes.
- Eight genes showed differential expression with increased ozone levels.
- Co-expression analysis revealed functional gene clustering related to ozone tolerance, particularly in LGs 5, 8, and 10.
Conclusions:
- Identified novel metabolic and defense pathways involved in conifer ozone tolerance.
- Provides a foundation for developing molecular strategies to enhance ozone resistance in peri-urban Abies religiosa forests.
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