Comparison of the modeling effects between two hemorrhoid models.

Area of Science:

• Biomedical Research
• Animal Models
• Gastroenterology

Background:

• Hemorrhoids are a common condition requiring effective treatment models.
• Existing models may not fully replicate human hemorrhoid pathology.

Purpose of the Study:

• To compare the efficacy of a novel rat internal hemorrhoid model against a standard croton oil-induced model.
• To determine which model better reflects clinical hemorrhoid features for research.

Main Methods:

• 48 rats were divided into control and model groups.
• A novel model combined croton oil with specific posture and movement; the control used croton oil alone.
• Evaluations included clinical signs, anorectal coefficient, histopathology, laser speckle imaging, RT-qPCR, IHC, and Western blotting.

Main Results:

• The novel model showed increased swelling, rectal prolapse, and anorectal coefficient compared to controls.
• Histopathology revealed significant cell death and vascular congestion in the novel model.
• Increased rectal blood perfusion, elevated pro-inflammatory cytokine mRNA (IL-2, IL-6, TNFα), and protein levels were observed in the novel model.

Conclusions:

• The novel rat internal hemorrhoid model more accurately reflects clinical hemorrhoid characteristics.
• This model is superior for preclinical research and the development of hemorrhoid therapies.