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Related Experiment Video

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Protocol for isolating viable human central nervous system T cells.

Hendrik J Engelenburg1, Joost Smolders2, Inge Huitinga3

  • 1Neuroimmunology Research Group, Netherlands Institute for Neuroscience, Amsterdam 1105 BA, the Netherlands.

STAR Protocols
|March 26, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a fast method for isolating T cells from post-mortem human brain tissue across various compartments. This protocol enables immune surveillance research in the central nervous system (CNS).

Keywords:
Cell isolationFlow CytometryImmunologyNeuroscience

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Area of Science:

  • Neuroimmunology
  • Cellular Immunology
  • Human tissue research

Background:

  • Brain-resident T cells are crucial for immune surveillance and maintaining homeostasis within the central nervous system (CNS).
  • Understanding the role of these cells requires efficient methods for their isolation from human brain tissue.

Purpose of the Study:

  • To develop and present a rapid protocol for isolating viable T cells from post-mortem human brain tissue.
  • To enable downstream analysis of T cells from multiple CNS compartments and peripheral blood.

Main Methods:

  • Mechanical and enzymatic tissue dissociation of post-mortem human brain samples.
  • Density gradient centrifugation for isolating mononuclear cells.
  • Collection of cells from choroid plexus, leptomeninges, dura mater, cerebrospinal fluid, parenchyma, and peripheral blood.

Main Results:

  • A protocol for the rapid isolation of viable T cells from diverse post-mortem human CNS compartments was established.
  • The method allows for the simultaneous collection of matched peripheral blood samples.

Conclusions:

  • This protocol provides a valuable tool for researchers studying T cell function in the CNS.
  • It facilitates immune surveillance and homeostasis research using human post-mortem brain tissue.