Vibrio natriegens, a Promising Chassis from Strain Property to Protein Expression: A Review

Protein expression is an important aspect of synthetic biology, where host selection critically impacts the production efficiency. Vibrio natriegens (V. natriegens) has emerged as a promising prokaryotic host due to its exceptionally rapid growth rate and broad substrate metabolic spectrum. Benefiting from the advances in annotation on microbial genomes and metabolic pathways, as well as the development of molecular biology tools, continuous progress has been made in bioproduction using V. natriegens. In this Perspective, we provide a systematic introduction of the basic biological properties of V. natriegens and present the engineered V. natriegens chassis which are currently used for protein expression. We summarize the latest synthetic biology components and genetic tools specifically designed for the modification of V. natriegens. Especially, we focus on the most recent study and application achievements of V. natriegens in in vivo and in vitro protein expression, which has received limited attention in previous reviews. This review aims to present researchers with a comprehensive overview of using V. natriegens for protein expression, highlight its advantages and application prospects, and present some challenges and future perspectives in this field, so as to promote wider research in the synthetic biology field.