Klf5 Regulates the Transition from Apical Radial Glia to Intermediate Progenitor Cells in the Developing Mammalian...

Krüppel-like factors (Klfs) are DNA-binding transcriptional factors that regulate multiple physiological features, including the cell cycle, cell differentiation, and tissue organization. Among them, Klf2, Klf4, and Klf5 are crucial for the induction and maintenance of pluripotent stem cells. However, the roles of these factors in maintaining neural stem cells (NSCs) remain poorly understood. Here, we show that Klf5 plays a dominant role in maintaining neural precursor cell (NPC) populations by suppressing their differentiation and radial migration in developing mouse brains of either sex. Klf5 also regulates the proliferation of NPCs and promotes differentiation of Pax6⁺ apical radial glia in the ventricular zone to Eomes⁺ (Tbr2⁺) intermediate progenitor cells in the subventricular zone by upregulating Hes1 and Eomes expression. Overexpression of Klf5 in NPCs reduced the pool of quiescent NSCs in the postnatal brain, resulting in attenuated neurogenesis in the subependymal zone and the dentate gyrus of the hippocampus in the adult brain. Klf5-overexpressing male mice exhibited impaired memory formation and reduced preference for social novelty. Our findings suggest a mechanism by which NPCs expand the output of differentiating cells through intermediate progenitor populations.