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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
Published on: June 12, 2018
Linking miRNAs to decay
1Molecular Biology Department, School of Biological Sciences, University of California, San Diego, La Jolla, California 92093, USA apasquinelli@ucsd.edu.
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MicroRNAs (miRNAs) regulate gene expression by recruiting Argonaute (AGO) proteins to specific targets, forming the microRNA (miRNA)-induced silencing complex (miRISC). In this issue of Genes & Development, Grimme and colleagues (doi:10.1101/gad.353314.125) determine how a family of related miRNAs is coordinately downregulated through limited base-pairing interactions with a long noncoding RNA (lncRNA). The lncRNA serves as a potent trigger to induce target-mediated miRNA decay (TDMD) despite supporting a looser pairing architecture with the miRNAs than typically needed.
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