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Published on: December 1, 2023
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The Impact of Dentin Primers on Collagen Secondary Structure: An Infrared Spectroscopic Analysis Using Amide III
Konstantinos Anastasiadis1, Oikonomou Michael1
1Private Practice, Athens, Greece.
Summary
Dentin primers impact collagen structure differently. HEMA-based primers disrupt collagen, while self-etch systems preserve it, influencing restoration durability.
Area of Science:
- Biomaterials Science
- Dental Materials
- Biochemistry
Background:
- Collagen secondary structure is crucial for dentin's mechanical properties.
- Dentin primers and etching strategies can alter collagen conformation.
- Understanding these alterations is key to improving adhesive dentistry.
Purpose of the Study:
- To evaluate the effects of different dentin primers and etching strategies on human dentin collagen secondary structures.
- To correlate demineralization extent with collagen structural changes.
Main Methods:
- Six primers (etch-and-rinse and self-etch) were applied to human dentin.
- Attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy analyzed collagen structure.
- Demineralization was quantified, and collagen secondary structure changes were assessed.
Main Results:
- Etch-and-rinse methods caused more demineralization than self-etch.
- HEMA, MP3M, and SEBO primers altered collagen structure (reduced alpha-helix, increased beta-sheet).
- MIP, DECO, and SECU primers largely preserved collagen conformation.
Conclusions:
- Dentin primer composition significantly affects collagen secondary structure.
- HEMA-rich primers were disruptive, while polyacrylic acid- and 4-META-based self-etch systems preserved structure.
- Demineralization extent correlates with collagen unfolding, informing future primer design for enhanced bond durability.
Keywords:
Fourier transform infrared spectroscopycollagendental adhesivesdentindentin primersfunctional monomersMore Related Videos
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