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All-Dielectric Metasurface-Enhanced Fluorescence for Probing Mitochondrial Membrane Potential Dynamics.

Xiaoqing Luo1, Qian Yuan2,3, Rong Shi4

  • 1CAS Key Laboratory of Human-Machine Intelligence-Synergy Systems, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Chemical & Biomedical Imaging
|March 27, 2026
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Summary
This summary is machine-generated.

We developed a novel fluorescence probe enhanced by an all-dielectric metasurface to monitor mitochondrial membrane potential (MMP). This technology significantly boosts fluorescence signals, enabling sensitive detection of cellular health changes.

Keywords:
all-dielectric metasurfacebiosensorbound state in the continuumbrillouin zone foldingfluorescence probemitochondrial

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cellular Biology

Background:

  • Fluorescence probes are crucial for assessing mitochondrial membrane potential (MMP), a vital marker of cellular health.
  • All-dielectric metasurfaces offer promising, yet underexplored, potential for enhancing fluorescence biosensing applications.

Purpose of the Study:

  • To demonstrate a novel fluorescence probe for monitoring mitochondrial membrane potential (MMP) dynamics.
  • To leverage an all-dielectric metasurface to significantly enhance the sensitivity of fluorescence-based cellular health monitoring.

Main Methods:

  • Designed an all-dielectric metasurface supporting bound states in the continuum (BICs) for high quality factor and near-field enhancement.
  • Utilized the metasurface to enhance fluorescence signals from a probe monitoring mitochondrial membrane potential (MMP).
  • Experimentally compared fluorescence intensity from cells cultured on and off the metasurface.

Main Results:

  • Achieved an order-of-magnitude enhancement in fluorescence intensity for cells on the metasurface compared to those off it.
  • Demonstrated the metasurface's effectiveness irrespective of forskolin stimulation, indicating broad applicability.
  • Showcased the ability to dynamically monitor cellular health via MMP fluctuations at subthreshold concentrations.

Conclusions:

  • All-dielectric metasurfaces can dramatically enhance fluorescence probes for monitoring mitochondrial membrane potential (MMP).
  • This enhanced sensing approach enables sensitive, dynamic assessment of cellular health and mitochondrial function.
  • The developed technology holds promise for advanced biosensing and diagnostics.