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Related Experiment Video

Updated: Mar 28, 2026

Identification of Coding and Non-coding RNA Classes Expressed in Swine Whole Blood
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Transcriptomic data of piglet blood compartments with 3' mRNA sequencing.

Gaëlle Payros1, Kory Jason1, Laure Gress2

  • 1Toxalim, Université de Toulouse, INRAE, ENVT, EI-Purpan, Toulouse, France.

Data in Brief
|March 27, 2026
PubMed
Summary
This summary is machine-generated.

This study presents new transcriptomic data from porcine blood, plasma, and serum using 3' mRNA-Seq. The method successfully profiles RNA from various sample types, including exosomes, offering valuable insights for research.

Keywords:
3′ mRNA-SeqExosomesPigPlasmaSerumTranscriptomeWhole blood

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Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Transcriptomic analysis provides insights into gene expression.
  • 3' mRNA-Seq is a method for sequencing the 3' ends of messenger RNAs.
  • Porcine models are relevant for biomedical and veterinary research.

Purpose of the Study:

  • To generate and report transcriptomic datasets from porcine samples using 3' mRNA-Seq.
  • To assess the applicability of 3' mRNA-Seq across different biological compartments, including whole blood, plasma, serum, and exosomes.
  • To provide a comprehensive transcriptomic resource for future research.

Main Methods:

  • Rationalized sequencing of the 3' ends of messenger RNAs (3' mRNA-Seq) was performed.
  • Libraries were prepared from whole blood, plasma, serum, and exosome-enriched fractions.
  • Porcine globin blockers were used for whole blood samples to minimize globin transcript interference.

Main Results:

  • Successful generation of transcriptomic libraries from whole blood, plasma, serum, and exosome-enriched fractions.
  • Distinctive transcriptomic profiles were observed between plasma/serum and whole blood samples.
  • A significant proportion of genes were detected in plasma and serum extracts, demonstrating the utility of 3' mRNA-Seq for these biofluids.

Conclusions:

  • 3' mRNA-Seq is a viable method for generating transcriptomic data from various porcine sample types, including minimally invasive biofluids.
  • The generated dataset offers valuable transcriptomic information from multiple compartments.
  • The study supports the further exploration of 3' mRNA-Seq in biomedical and veterinary research.