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Transcriptomic data of piglet blood compartments with 3' mRNA sequencing
Gaëlle Payros1, Kory Jason1, Laure Gress2
1Toxalim, Université de Toulouse, INRAE, ENVT, EI-Purpan, Toulouse, France.
Data in Brief
|March 27, 2026
Summary
This study presents new transcriptomic data from porcine blood, plasma, and serum using 3' mRNA-Seq. The method successfully profiles RNA from various sample types, including exosomes, offering valuable insights for research.
Area of Science:
- Molecular Biology
- Genomics
- Bioinformatics
Background:
- Transcriptomic analysis provides insights into gene expression.
- 3' mRNA-Seq is a method for sequencing the 3' ends of messenger RNAs.
- Porcine models are relevant for biomedical and veterinary research.
Purpose of the Study:
- To generate and report transcriptomic datasets from porcine samples using 3' mRNA-Seq.
- To assess the applicability of 3' mRNA-Seq across different biological compartments, including whole blood, plasma, serum, and exosomes.
- To provide a comprehensive transcriptomic resource for future research.
Main Methods:
- Rationalized sequencing of the 3' ends of messenger RNAs (3' mRNA-Seq) was performed.
- Libraries were prepared from whole blood, plasma, serum, and exosome-enriched fractions.
- Porcine globin blockers were used for whole blood samples to minimize globin transcript interference.
Main Results:
- Successful generation of transcriptomic libraries from whole blood, plasma, serum, and exosome-enriched fractions.
- Distinctive transcriptomic profiles were observed between plasma/serum and whole blood samples.
- A significant proportion of genes were detected in plasma and serum extracts, demonstrating the utility of 3' mRNA-Seq for these biofluids.
Conclusions:
- 3' mRNA-Seq is a viable method for generating transcriptomic data from various porcine sample types, including minimally invasive biofluids.
- The generated dataset offers valuable transcriptomic information from multiple compartments.
- The study supports the further exploration of 3' mRNA-Seq in biomedical and veterinary research.
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