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SNED1 modulates ECM architecture and cell proliferation via LDV-binding integrins
Dharma Pally1, Leanna Leverton1, Asantewaa Jones1
1Department of Physiology and Biophysics, University of Illinois Chicago, Chicago, IL, 60612, U.S.A.
Biorxiv : the Preprint Server for Biology
|March 27, 2026
Summary
SNED1 protein interaction with LDV-binding integrins, not RGD-binding integrins, is crucial for extracellular matrix (ECM) assembly and remodeling. This interaction influences cell proliferation and alignment, impacting development and disease.
Area of Science:
- Biochemistry
- Cell Biology
- Developmental Biology
Background:
- The extracellular matrix (ECM) provides structural support and regulates cell behavior through interactions with cell surface receptors like integrins.
- Understanding ECM assembly and its regulation by cell-matrix interactions is vital for comprehending diseases such as cancer and fibrosis.
- SNED1 is a novel ECM protein implicated in development and metastasis, but its assembly and signaling mechanisms are poorly understood.
Purpose of the Study:
- To investigate the role of SNED1/integrin interactions in the assembly and remodeling of the SNED1-containing ECM.
- To determine whether SNED1's RGD and LDV integrin-binding motifs differentially regulate ECM formation and cellular responses.
Main Methods:
- Investigated SNED1/integrin interactions using cell adhesion assays.
- Analyzed ECM assembly, protein patterning (SNED1, fibronectin, collagen I), and ECM/cell alignment through microscopy.
- Assessed the impact of SNED1/integrin interactions on cell proliferation.
Main Results:
- SNED1 incorporation into the ECM did not require integrin interaction.
- Interaction with LDV-binding integrins, but not RGD-binding integrins, was essential for SNED1 ECM build-up and patterning of ECM proteins.
- SNED1/LDV-integrin interaction promoted ECM alignment, cell alignment, and cell proliferation.
Conclusions:
- SNED1 interaction with LDV-binding integrins, distinct from RGD-binding integrins, is critical for ECM remodeling.
- This interaction regulates cytoskeletal rearrangement and cell proliferation, influencing processes like neural crest cell migration and cancer invasion.
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