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Area of Science:

  • Metabolism and Endocrinology
  • Cellular Biology

Background:

  • Beige adipocytes in subcutaneous white adipose tissue (sWAT) are crucial for thermogenesis during cold exposure.
  • Rho-associated coiled-coil containing kinase (ROCK) inhibitors can influence white adipocyte differentiation.
  • The specific role of ROCK2, the main ROCK isoform in adipocytes, in cold-induced thermogenesis remains unclear.

Purpose of the Study:

  • To investigate the adipocyte-specific function of ROCK2 in cold-induced thermogenesis.
  • To elucidate the molecular mechanisms by which ROCK2 influences adaptive thermogenesis.

Main Methods:

  • Utilized mice with adipocyte-specific ROCK2 deficiency.
  • Analyzed cold-induced thermogenic responses in sWAT and interscapular brown adipose tissue (iBAT).
  • Examined the role of FOXO1 and Ucp1 expression in ROCK2-mediated thermogenesis.

Main Results:

  • ROCK2 activation in adipocytes is rapidly induced by cold exposure in wild-type mice.
  • Adipocyte-specific ROCK2 deficiency impairs cold-induced beige adipocyte development in sWAT and reduces thermogenesis in iBAT.
  • ROCK2 mediates cold-induced Ucp1 expression in sWAT via FOXO1 upregulation and nuclear translocation.

Conclusions:

  • ROCK2 in adipocytes is indispensable for adaptive beiging and thermogenesis in response to cold.
  • ROCK2 plays a critical role in regulating energy homeostasis through its influence on thermogenic processes.
  • Targeting ROCK2 may offer therapeutic strategies for metabolic disorders related to energy balance.