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Updated: Mar 29, 2026

In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
Published on: December 29, 2021
The Journey of Histones: Molecular Mechanisms of the Histone Chaperoning Cascade
Carol Cho1, Ji-Joon Song1,2
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Korea.
Abstract:
In eukaryotes, genomic DNA is packaged into hierarchical higher-order chromatin structures. Regulation of this organization is essential for the faithful maintenance and controlled expression of genetic information. Assembly of the nucleosome─the fundamental unit of chromatin─depends on a tightly coordinated network of histone chaperones that escort histones from their initial synthesis through multiple intermediates to their final deposition into nucleosomes. This review provides an overview of the stepwise journey of histones and the regulatory mechanisms that govern this process. We also highlight major outstanding questions and discuss future directions in the field.
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