Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

997
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
997
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.0K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hybrid solid-liquid optics enable scalable, high-resolution light-sheet microscopy across diverse immersion media.

Nature biotechnology·2026
Same author

Lateral entorhinal cortex supports behaviorally-induced hippocampal ensemble stability for reliable memory recall.

bioRxiv : the preprint server for biology·2026
Same author

Just Go with the Optical Flow: Enhanced Axon Tracing by Modeling Fiber Connections as Motion.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

Hybrid Solid-Liquid Optics Enable Scalable, High-Resolution, Multi-Immersion Light-Sheet Microscopy.

bioRxiv : the preprint server for biology·2025
Same author

Enhanced Distal Signaling in Human Hippocampal Neurons despite Lower Intrinsic Excitability.

Research square·2025
Same author

Cortical glutamatergic and GABAergic inputs support learning-driven hippocampal stability.

Science (New York, N.Y.)·2025
Same journal

Human-AI Interaction in Interventional Radiology: A Narrative Review of Current Applications, Challenges, and Future Directions.

Journal of imaging·2026
Same journal

Coronary Artery Anomalies and Anatomical Variants: Cross-Sectional Diagnostic Imaging and Clinical Background.

Journal of imaging·2026
Same journal

YoLeTooth: A Unified Framework for Joint Tooth Segmentation and Periapical Lesion Detection in Panoramic Radiographs.

Journal of imaging·2026
Same journal

Radiomics-Guided Multi-Sequence Learning for Pathological Complete Response Prediction from Breast MRI with Missing Auxiliary Sequences.

Journal of imaging·2026
Same journal

Cutaneous Thermography in Arthropathies: Quantitative Imaging, Machine Learning, and Clinical Translation.

Journal of imaging·2026
Same journal

Two-Stage Dynamic Synergistic Segmentation Method for Myocardial Pathology.

Journal of imaging·2026
See all related articles

Related Experiment Video

Updated: Mar 29, 2026

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.7K

ClearScope: A Fully Integrated Light-Sheet Theta Microscope for Sub-Micron-Resolution Imaging Without Lateral Size

Matthew G Fay1, Peter J Lang1, David S Denu1

  • 1MBF Bioscience, Williston, VT 05495, USA.

Journal of Imaging
|March 27, 2026
PubMed
Summary
This summary is machine-generated.

A new light-sheet theta microscopy (LSTM) system enables high-resolution 3D imaging of large, cleared brain tissues. This technology facilitates detailed mapping of neural connectivity and cellular features in both animal models and human samples.

Keywords:
connectomicslight-sheet microscopylight-sheet theta microscopyneuroscience

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.2K
Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development
08:37

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Published on: May 5, 2014

23.8K

Related Experiment Videos

Last Updated: Mar 29, 2026

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.7K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.2K
Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development
08:37

Setting Up a Simple Light Sheet Microscope for In Toto Imaging of C. elegans Development

Published on: May 5, 2014

23.8K

Area of Science:

  • Neuroscience
  • Biomedical Imaging
  • Microscopy

Background:

  • 3D ex vivo imaging of cleared brain tissue is crucial for understanding neural connectivity and neurological disorders.
  • Current light-sheet microscopy methods are limited by specimen size constraints due to orthogonal illumination and detection.
  • Light-sheet theta microscopy (LSTM) offers a solution by using oblique illumination paths.

Purpose of the Study:

  • To develop and present a next-generation, integrated, and user-friendly light-sheet theta microscopy (LSTM) system.
  • To enable uniform subcellular-resolution 3D imaging of large, cleared specimens without lateral dimension constraints.
  • To provide a seamless workflow for image acquisition, processing, and quantitative analysis.

Main Methods:

  • Development of a fully integrated and user-friendly light-sheet theta microscopy (LSTM) system.
  • Utilizing two oblique illumination paths with perpendicular detection geometry.
  • Implementing a workflow for image acquisition, data storage, pre- and post-processing, enhancement, and quantitative analysis.

Main Results:

  • Demonstrated uniform subcellular-resolution 3D imaging throughout large specimens.
  • Successfully imaged intact mouse brains and human brain samples.
  • Performed complete downstream analyses including digital neuron tracing, vascular reconstruction, and stereological analysis.

Conclusions:

  • The enhanced LSTM system overcomes previous specimen size limitations.
  • This accessible technology enables rapid, quantitative mapping of molecular and cellular features in large biological samples.
  • Facilitates advanced research into neurological and neuropsychiatric disorders through detailed brain imaging.