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New Insights from Two Historic Boletellus-Type Specimens in China Based on Next-Generation Sequencing.
Bing-Qian Yang1,2,3, Hong-Kang Shen2,3,4, Yang Wang2,3
1School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Department of Pharmaceutical Sciences, Kunming Medical University, Kunming 650500, China.
Journal of Fungi (Basel, Switzerland)
|March 27, 2026
Summary
This study reassesses historical Chinese Boletellus species using genome skimming. Findings reveal B. serpentipileus belongs to Leccinum and B. vulgaris to Austroboletus, clarifying bolete taxonomy in China.
Area of Science:
- Mycology
- Taxonomy
- Genomics
Background:
- The Boletellus genus is morphologically distinct within Boletaceae.
- Historically described Chinese Boletellus species have been understudied.
- Accurate classification of fungal species is crucial for biodiversity studies.
Purpose of the Study:
- To clarify the taxonomic identities of historical Chinese Boletellus species.
- To re-evaluate the placement of B. serpentipileus and B. vulgaris.
- To contribute to a more accurate understanding of bolete diversity in China.
Main Methods:
- Genome skimming (next-generation sequencing) of historical type specimens.
- Sanger sequencing for targeted gene analysis.
- Morphological re-examination of fungal specimens.
Main Results:
- B. serpentipileus was reclassified into the genus Leccinum as L. serpentipileum.
- B. vulgaris was reclassified into the genus Austroboletus and synonymized with A. fusisporus.
- The study resolved long-standing taxonomic uncertainties for these species.
Conclusions:
- B. serpentipileus and B. vulgaris are not true members of the Boletellus genus.
- The findings necessitate taxonomic revisions within Boletaceae.
- This research enhances the accuracy of fungal biodiversity records in China.
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