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The TCP transcription factor RmTCP5 shapes leaf development by modulating auxin and cytokinin homeostasis in Rosa
Wan Zhu1, Ying Bao2, Huijia Cao1
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, 430070, China.
Journal of Plant Physiology
|March 27, 2026
Summary
Researchers identified RmTCP5, a key gene regulating rose leaf shape. Overexpression altered leaf morphology and affected auxin/cytokinin pathways, revealing conserved TCP gene functions in plants.
Area of Science:
- Plant Biology
- Genetics
- Molecular Biology
Background:
- Leaf morphology is crucial for plant development and adaptation.
- TCP transcription factors are vital regulators of plant growth and leaf shape.
- Understanding TCP gene function in the genus Rosa is limited.
Purpose of the Study:
- To investigate the role of the CIN-clade TCP gene, RmTCP5, in regulating leaf morphology in Rosa multiflora.
- To explore the molecular mechanisms underlying RmTCP5's function in leaf development.
Main Methods:
- Functional analysis of RmTCP5 in heterologous (Arabidopsis, tomato) and homologous (rose) systems.
- Transcriptome profiling of transgenic plants to identify affected gene expression.
- Yeast one-hybrid (Y1H) and dual-luciferase reporter (DLR) assays to confirm gene interactions.
Main Results:
- Overexpression of RmTCP5 caused significant changes in leaf shape, including reduced area and altered length/width ratio.
- Transcriptome analysis revealed impacts on auxin and cytokinin signaling pathways.
- RmTCP5 was shown to bind and suppress the expression of RhYUC5 and RhCKX5 genes.
Conclusions:
- RmTCP5 is a key regulator of leaf morphology in Rosa multiflora.
- The findings highlight the conserved role of TCP genes in leaf development across species.
- This study provides novel insights into the specific functions of TCP genes in rose.
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