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Thermosensory reconfiguration of the auxin transcriptional pathway to drive root cell growth
María Belén Borniego1, Matías Ezequiel Pereyra1,2, Katelyn Sageman-Furnas3,4
1Facultad de Agronomía, Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura (IFEVA), Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
Abstract:
Elevated temperatures enhance plant root growth. We find that cell elongation significantly contributes to this response. While mutations in the auxin transcriptional pathway impair warmth-induced cell elongation, exogenous auxin inhibits this growth. Intriguingly, warmth increases auxin levels and the nuclear accumulation of TIR1/AFB2/AFB3, alongside Aux/IAA stabilisation. This apparent paradox is explained by the concurrent increase in nuclear AFB1, which stabilises Aux/IAAs and promotes cell elongation. Notably, despite enhancing Aux/IAA stability, warmth also promotes ARF transcriptional activity. ARF7/19 are essential for warmth-induced cell growth and its inhibition by exogenous auxin. Warmth directly modulates ARF7/19 by reducing oligomerisation and cytoplasmic condensation, thereby enhancing their nuclear accumulation. This mechanism effectively repurposes the auxin pathway to regulate root cell growth in response to temperature.
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Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
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