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Updated: Mar 29, 2026

Transient Expression in Nicotiana Benthamiana Leaves for Triterpene Production at a Preparative Scale
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Exogenous Methyl Jasmonate Promotes Triterpene Accumulation in Loquat Callus.

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Summary

Methyl jasmonate (MeJA) treatment significantly boosts triterpene accumulation in loquat callus. This study reveals key genes and pathways involved in MeJA-induced terpenoid biosynthesis, offering insights for pharmaceutical applications.

Keywords:
EjCYP716AEjOSCjasmonic acid (JA) signaling and JA responseloquattriterpene metabolites

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Area of Science:

  • Plant Biotechnology
  • Plant Biochemistry
  • Metabolomics

Background:

  • Loquat (Eriobotrya japonica) is rich in bioactive triterpenes with pharmaceutical value.
  • Traditional Chinese Medicine utilizes loquat leaves, highlighting its medicinal potential.
  • Mechanisms of MeJA-induced terpenoid biosynthesis in loquat callus are not well understood.

Purpose of the Study:

  • To investigate the effect of Methyl Jasmonate (MeJA) on terpenoid biosynthesis in loquat callus.
  • To identify differential metabolites and key genes regulated by MeJA treatment.
  • To elucidate the regulatory mechanisms of MeJA-induced terpenoid accumulation.

Main Methods:

  • Integrated targeted metabolomic and transcriptomic analysis.
  • Treatment of loquat callus with exogenous MeJA.
  • Identification and quantification of terpenoid compounds and gene expression profiling.

Main Results:

  • MeJA treatment significantly increased the accumulation of 131 terpenoid compounds, primarily triterpenes.
  • Ursolic acid and betulinic acid showed the highest accumulation at different time points (24h and 48h).
  • Upregulation of key terpenoid biosynthesis genes (e.g., EjFPSs, EjSQEs) and JA-signaling pathway genes was observed.

Conclusions:

  • MeJA effectively induces terpenoid biosynthesis in loquat callus.
  • The study provides a mechanistic understanding of MeJA's role in regulating terpenoid production.
  • Establishes a foundation for using plant cell culture for producing valuable terpenoids for food and pharmaceutical industries.