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Updated: Mar 29, 2026
09:36
RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
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Functional Characterization of Long Non-Coding RNAs Associated with Reproductive Fitness in Pura Raza Española Mares
María Ángeles Vargas-Pérez1, Nora Laseca2, Sebastián Demyda-Peyrás1
1Departamento de Genética, Universidad de Córdoba, 14071 Córdoba, Spain.
Animals : an Open Access Journal From MDPI
|March 28, 2026
Summary
Long non-coding RNAs (lncRNAs) may regulate horse fertility by forming DNA:RNA triplexes with gene promoter regions. This study identified potential lncRNAs involved in fertility in Pura Raza Española horses.
Area of Science:
- Genomics
- Molecular Biology
- Animal Reproduction
Background:
- Long non-coding RNAs (lncRNAs) are key gene expression regulators.
- lncRNAs are implicated in equine female reproductive fitness, but mechanisms are unclear.
Purpose of the Study:
- Investigate DNA:RNA triplex interactions between fertility-related gene promoters and nearby lncRNAs in Pura Raza Española (PRE) horses.
- Elucidate regulatory mechanisms of equine fertility.
Main Methods:
- Bioinformatic analysis of lncRNAs and promoter regions within ±50 kb.
- Genomic context and structural accessibility analyses.
- Identification of potential DNA:RNA triplex formation sites.
Main Results:
- Identified lncRNAs located in cis (1.2-49.8 kb) to fertility genes.
- Some predicted DNA-binding sites are within CpG islands of active promoters.
- Potential for lncRNAs to modulate transcription via triplex formation.
Conclusions:
- Identified plausible lncRNAs regulating fertility in PRE horses.
- These lncRNAs represent targets for functional validation.
- Potential applications in equine genomic improvement strategies.
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