Related Experiment Video
Updated: Mar 29, 2026
Investigation of Beige Fat Biology and Metabolism Using the CRISPR SunTag-p65-HSF1 Activation System
Published on: January 6, 2023
ARHGDIB Modulates Subcutaneous Fat Deposition in Ducks
Mingyu Wang1, Hao Zheng1, Xing Chen2
1Laboratory of Genetic Breeding, Reproduction and Precision Livestock Farming, School of Animal Science and Nutritional Engineering, Wuhan Polytechnic University, Wuhan 430023, China.
Background:
Subcutaneous fat deposition critically impacts duck meat quality and feed efficiency. ARHGDIB, a Rho GTPase regulator implicated in adipogenesis, remains functionally uncharacterized in poultry.
Methods:
We monitored growth and fat deposition in ducks from 30 to 70 days, performed transcriptomics on adipose tissue, and established an in vitro duck preadipocyte model to assess ARHGDIB function via siRNA knockdown, Oil Red O staining, and RNA-seq.
Results:
Fat deposition peaked at 50 days. ARHGDIB expression was lowest in fat and decreased during differentiation. Its knockdown significantly enhanced lipid accumulation, upregulated PPARγ and LPL, and altered the expression of 1681 genes enriched in oxidative phosphorylation, insulin/TLR signaling, and autophagy pathways.
Conclusions:
ARHGDIB acts as a novel negative regulator of duck subcutaneous adipogenesis by suppressing differentiation and modulating an integrated metabolic-inflammatory network, offering a potential target for precision breeding.
