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Preparation of 3D Decellularized Matrices from Fetal Mouse Skeletal Muscle for Cell Culture
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MSC Origin and Biomechanical Conditioning Determine ECM Maturation in Tissue-Engineered Matrix.
Michelle Klein1,2,3,4, Arian Ehterami1, Neguin Ranjbar1,5
1Institute for Regenerative Medicine (IREM), University of Zurich, Wagistrasse 12, 8952 Schlieren, Switzerland.
Biomedicines
|March 28, 2026
Summary
Hydrodynamic stimulation enhances tissue-engineered matrix (TEM) development by improving extracellular matrix (ECM) deposition and collagen maturation. Umbilical cord-derived mesenchymal stromal cells (MSCs) showed the most advanced collagen maturation, while adipose-derived MSCs led to greater matrix thickening.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Cell Biology
Background:
- The extracellular matrix (ECM) is crucial for the mechanical strength of tissue-engineered matrices (TEMs), especially for cardiovascular and load-bearing applications.
- Mesenchymal stromal cells (MSCs) from different sources exhibit varying potentials for ECM formation.
- Understanding these differences is key to developing effective TEMs.
Purpose of the Study:
- To compare the ECM-forming potential of human adipose-derived (hADMSC), bone marrow-derived (hBMSC), and umbilical cord-derived MSCs (hUCMSC) for TEM development.
- To evaluate the impact of static versus hydrodynamic culture conditions on TEM characteristics.
- To identify optimal cell sources and culture methods for clinically relevant TEMs.
Main Methods:
- Cells (hADMSC, hBMSC, hUCMSC, and human dermal fibroblasts as reference) were seeded onto PGA/P4HB scaffolds.
- Cultures were maintained for 3 weeks under static or hydrodynamic (orbital shaking) conditions.
- TEM development was assessed via macroscopic, histological, polarized light microscopy, and biochemical assays (DNA, GAGs, hydroxyproline).
Main Results:
- Hydrodynamic stimulation consistently enhanced ECM deposition and collagen maturation across all cell types.
- MSC-derived TEMs showed increased matrix thickening and more uniform ECM distribution under hydrodynamic conditions compared to static cultures.
- Human dermal fibroblast-derived TEMs had higher total collagen but remained thinner; hUCMSC-derived TEMs exhibited superior collagen maturation and distribution.
Conclusions:
- Both cell source and hydrodynamic stimulation are critical for optimizing ECM deposition and collagen maturation in TEMs.
- hUCMSC-derived TEMs demonstrated advanced collagen maturation and uniform distribution under dynamic culture.
- hADMSC-derived TEMs showed significant matrix thickening, highlighting the influence of cell source on TEM volumetric expansion and maturation.
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