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Physiologically Relevant Organotypic Tissue Slice Model for Evaluating Cell Responses to Ionizing Radiation.

Victoria Shestakova1,2, Ekaterina Smirnova1,2, Elena Isaeva1

  • 1National Medical Research Radiological Center of the Ministry of Health of the Russian Federation, Koroleva st. 4, 249036 Obninsk, Russia.

International Journal of Molecular Sciences
|March 28, 2026
PubMed
Summary

Precision-cut organotypic tissue slices (OTSs) offer a standardized model for assessing radiation therapy effects. This approach enhances the reliability of radiobiological studies, advancing radiation medicine.

Keywords:
cell deathcell viabilityionizing radiationorganotypic tissue cultureorganotypic tissue slicesradiation therapyradiobiologytissue engineering

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Area of Science:

  • Radiotherapy and Radiation Oncology
  • Biomedical Engineering
  • Cellular and Molecular Biology

Background:

  • Precision radiotherapy demands standardized, reproducible, and biologically relevant models for evaluating radiobiological sources.
  • Current models like in vitro cell cultures and in vivo animal models have limitations in mimicking in vivo complexity.
  • A unified approach is needed to bridge the gap between current in vitro and in vivo models.

Purpose of the Study:

  • To introduce and review precision-cut organotypic tissue slices (OTSs) as a novel, representative model for assessing radiobiological sources.
  • To highlight the potential of OTSs in unifying the assessment of radiation efficacy and safety.
  • To discuss the application of OTSs in advancing radiation medicine and research.

Main Methods:

  • Utilizing precision-cut organotypic tissue slices (OTSs) derived from specific organs.
  • Retaining complex tissue architecture and multicellular environments within the OTS model.
  • Employing emerging practices for OTS preparation, radiation exposure, and outcomes assessment.

Main Results:

  • OTSs effectively mimic in vivo physiological responses to ionizing radiation.
  • The model provides insights into radiation-induced damage, repair mechanisms, and potential toxicities.
  • Standardized OTS preparation improves the reliability and comparability of radiobiological studies.

Conclusions:

  • Organotypic tissue slices (OTSs) offer a physiologically relevant platform for radiobiological research.
  • This approach facilitates the development of safer and more effective radiation therapies.
  • OTSs hold significant potential to advance our understanding and application of radiation medicine.