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Updated: Mar 29, 2026

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Downregulation of the Long Non-Coding RNA MDL1AS Alters Metabolism, Differentiation, and Radiosensitivity in NTERA2

Adrián Casas-Benito1, Pablo Garrido1, Alfredo Martínez1

  • 1Angiogenesis Group, Oncology Area, Center for Biomedical Research of La Rioja (CIBIR), 26006 Logroño, Spain.

Cancers
|March 28, 2026
PubMed

Insights

Reducing the long non-coding RNA MDL1AS impacts cancer cell radiosensitivity and metabolism. This suggests MDL1AS could be a biomarker for new cancer therapies.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
  • MDL1AS is a novel lncRNA with potential involvement in cancer development.

Purpose of the Study:

  • To investigate the functional role of MDL1AS in cancer cells with different differentiation states.
  • To understand how MDL1AS impacts cellular behavior, metabolism, and response to therapy.

Main Methods:

  • Downregulation of MDL1AS in NTERA2 and SH-SY5Y cell lines.
  • Analysis of cellular behavior, oxidative phosphorylation, differentiation, proliferation, and DNA damage response.
  • RNA sequencing to analyze gene expression changes and apoptotic signatures.
  • Assessment of radiosensitivity.

Main Results:

  • MDL1AS reduction inhibited oxidative phosphorylation in NTERA2 cells and induced neuritic differentiation in SH-SY5Y cells.
  • Downregulation led to a strong DNA damage response and increased apoptosis in both cell lines.
  • Proliferation decreased, and radiosensitivity was reduced in NTERA2 cells but not SH-SY5Y cells.

Conclusions:

  • MDL1AS downregulation modulates radiosensitivity, metabolism, and differentiation in a cell-type-specific manner.
  • MDL1AS may serve as a predictive biomarker for cancer therapies.
  • MDL1AS represents a potential molecular target for novel therapeutic strategies.
Keywords:
DDRLamin B1MDL1ASNTERA2PAI-1SH-SY5Ydifferentiationglycolysismetabolismradiation sensitivity

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