Related Experiment Video

Updated: Aug 15, 2026

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
07:30

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons

Published on: May 12, 2015

A general method for the specific staining of intracellular antigens with ferritin-antibody conjugates

J D McLean, S J Singer

    Proceedings of the National Academy of Sciences of the United States of America
    |January 1, 1970
    PubMed

    A general method is described whereby an intracellular macromolecule can be specifically stained with its antibody conjugated to ferritin. In this method cells or cell organelles are fixed, embedded in bovine serum albumin cross-linked with glutaraldehyde or formaldehyde, and then sectioned. This procedure preserves antigenic determinants as well as the cellular ultrastructure. The application of the ferritin-antibody conjugate to the section produces specific staining of the exposed antigen.

    More Related Videos

    Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue08:37

    Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue

    Published on: May 28, 2015

    Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts09:21

    Ferritinophagy: Assessing the Selective Degradation of Iron by Autophagy in Human Fibroblasts

    Published on: February 23, 2024

    Related Concept Videos

    Immunofluorescence Microscopy01:12

    Immunofluorescence Microscopy

    A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
    The...
    Immunocytochemistry and Immunohistochemistry01:22

    Immunocytochemistry and Immunohistochemistry

    Immunocytochemistry (ICC) and immunohistochemistry (IHC) are techniques that use antibodies to check for specific proteins or antigens in a sample. The technique was first published by Albert Coons in 1941 to detect the presence of pneumococcal antigen in tissue sections from mice infected with Pneumococcus. Immunocytochemistry helps localization of proteins or antigens in individual cells like blood cells, stem cells, etc., while immunohistochemistry does the same for tissue samples.
    These...
    Immunogold Electron Microscopy01:20

    Immunogold Electron Microscopy

    Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
    Special Staining Techniques01:13

    Special Staining Techniques

    Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...