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Related Concept Videos

Methods for Studying Drug Absorption: In vitro01:16

Methods for Studying Drug Absorption: In vitro

755
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
755
Physiological Pharmacokinetic Models: Incorporating Hepatic Transporter-Mediated Clearance01:07

Physiological Pharmacokinetic Models: Incorporating Hepatic Transporter-Mediated Clearance

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Drug transporters are critical in drug absorption, distribution, and excretion processes. They should be included in physiological-based pharmacokinetic (PBPK) models, which help predict human drug disposition. However, predicting this is challenging during drug development, especially when liver transport is involved. However, with a realistic representation of body transport processes, an accurate model may be possible.
A recent model describes pravastatin's hepatobiliary excretion,...
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Methods for Studying Drug Absorption: In situ01:09

Methods for Studying Drug Absorption: In situ

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In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
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Cellular Membranes and Drug Transport01:24

Cellular Membranes and Drug Transport

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Drugs must traverse multiple biological barriers, such as multi-layered skin, single-layered intestinal epithelium, and the plasma membrane, to reach their target sites within the body. The plasma membrane, a highly structured composite of phospholipids, carbohydrates, and proteins, is the cell's protective boundary, facilitating selective substance exchange.
Phospholipids arrange themselves into a bilayer, with hydrophilic heads oriented outward and hydrophobic tails facing inward.
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Updated: Mar 29, 2026

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
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Integrated Experimental-Computational Framework for Drug Transport Quantification in 3D Microtissues™.

Ramisa Fariha1, Jad Hamze2, Oluwanifemi David Okoh1

  • 1Center for Biomedical Engineering, School of Engineering, Brown University, 182 Hope Street, Providence, RI 02912, USA.

Micromachines
|March 28, 2026
PubMed
Summary
This summary is machine-generated.

This study presents a new method for measuring drug absorption in 3D cancer models. The validated workflow accurately quantifies drug levels, aiding precision medicine and therapeutic optimization.

Keywords:
3D microtissuesLC–MS/MSdrug absorptionin vitro-to-in vivo extrapolationmicrophysiological systemsprecision medicinetissue engineering

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Area of Science:

  • Pharmacology
  • Biotechnology
  • Cancer Research

Background:

  • Traditional 2D cell cultures lack the complexity of in vivo tumor microenvironments.
  • 3D tissue culture systems, like Microtissues™, offer a more physiologically relevant alternative for drug screening.
  • Analyzing drug concentrations in 3D constructs presents significant analytical challenges.

Purpose of the Study:

  • To develop and validate an analytical method for quantifying drug absorption in 3D tissue models.
  • To establish a microscale tissue-engineered system for studying drug absorption and transport dynamics.
  • To enable in vitro-to-in vivo extrapolation (IVIVE) for improved therapeutic strategies.

Main Methods:

  • Utilized scaffold-free, high-throughput 3D Microtissues™ platform.
  • Developed an integrated workflow combining liquid-liquid extraction and protein precipitation.
  • Employed LC-MS/MS analysis for sensitive and accurate drug quantification.

Main Results:

  • Achieved a validated lower limit of quantification of 0.03 μM for paclitaxel.
  • Demonstrated robust assay linearity (R² ≥ 0.90) and precision (CV ≤ 10%).
  • Successfully quantified drug-tissue interactions in MCF7 breast cancer Microtissues™.

Conclusions:

  • The developed method enables precise characterization of drug absorption in microengineered 3D cancer models.
  • This platform supports IVIVE for therapeutic optimization and precision medicine applications.
  • Scalable and modular design facilitates the use of patient-derived microtissues for individualized treatment decisions.