Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

iChip01:24

iChip

62
The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
62
Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

3.5K
Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
3.5K
Need for Obtaining Pure Cultures01:29

Need for Obtaining Pure Cultures

2.6K
Pure cultures, defined as the growth of a single microorganism species isolated from mixed populations, are fundamental tools in microbiological research and practical applications. These cultures ensure genetic and physiological uniformity, allowing researchers to study microbial traits under controlled conditions.Isolation and Maintenance of Pure CulturesObtaining a pure culture involves isolating a single microbial type from a mixed sample through techniques such as serial dilutions, streak...
2.6K
Key Techniques in Microbiology01:19

Key Techniques in Microbiology

3.0K
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
3.0K
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

27
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
27
Bioreactor Controls-III01:22

Bioreactor Controls-III

51
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
51

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sludge Retention Time Governs Ectoine Synthesis and Pollutant Removal in Halophilic Activated Sludge Treating High-Salinity Wastewater.

Toxics·2026
Same author

Unit-specific fate and ecological drivers of antibiotic resistome in a full-scale swine wastewater treatment system.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Dissolved Fe(III) defines a stabilizing window for tetracycline resistance evolution under environmentally relevant conditions.

Water research·2026
Same author

Combating oxygen toxicity through antioxidants during anammox granular sludge preservation and mechanistic insights.

Bioresource technology·2026
Same author

Moderate thermal-chemical-enzymatic pretreatment for waste activated sludge: Methane yield enhancement and life cycle assessment.

Environmental research·2026
Same author

Seafood resistome across trophic levels: Tissue patterns, drivers, and potential dietary exposure.

Journal of hazardous materials·2026
Same journal

Correction: Peptine et al. Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Vancomycin-Resistant Enterococci (VRE) in Nosocomial Infections: A Systematic Review of Resistance, Pathogenesis, and Clinical Management. <i>Microorganisms</i> 2026, <i>14</i>, 428.

Microorganisms·2026
Same journal

Torque Teno Virus (TTV) Plasma Load and Immune Reconstitution in People Living with HIV: A Systematic Review.

Microorganisms·2026
Same journal

Optimizing Bacteriophage Screening and Isolation Methods for Microbial Samples Derived from Different Body Sites of Cattle.

Microorganisms·2026
Same journal

Enhanced Biphenyl Degradation by <i>Rhodococcus</i> sp. TG-1 Under Cr(VI) Stress via Modified Biochar Immobilization.

Microorganisms·2026
Same journal

In Vitro Detection of Biologically Active Staphylococcal Enterotoxins Type B and C1 as an Alternative to In Vivo Testing.

Microorganisms·2026
Same journal

Monitoring Hygiene Protocols and Exploring Alternatives to Counteract Resistant Pathogens: A Case Study from Southern Italy on Healthcare-Associated Infection Control.

Microorganisms·2026
See all related articles

Related Experiment Video

Updated: Mar 29, 2026

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC
08:18

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC

Published on: February 18, 2022

4.7K

Artificial Intelligence for Microbial Isolation and Cultivation: Progress and Challenges.

Mingyu Li1,2, Xiangwu Yao1,2, Meng Zhang1,2,3

  • 1State Key Laboratory of Soil Pollution Control and Safety, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China.

Microorganisms
|March 28, 2026
PubMed
Summary
This summary is machine-generated.

Artificial intelligence (AI) is revolutionizing microbial discovery by overcoming traditional cultivation limitations. This review details AI

Keywords:
artificial intelligenceculturomicsdeep learningmachine learningmicrobial isolation and cultivationmicrobial resource discovery

More Related Videos

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System
10:16

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System

Published on: March 14, 2025

1.5K
In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
11:07

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)

Published on: August 6, 2025

3.0K

Related Experiment Videos

Last Updated: Mar 29, 2026

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC
08:18

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System MMC

Published on: February 18, 2022

4.7K
Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System
10:16

Automated and High-throughput Microbial Monoclonal Cultivation and Picking Using the Single-cell Microliter-droplet Culture Omics System

Published on: March 14, 2025

1.5K
In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)
11:07

In Situ Isolation and Culturing of Recalcitrant Soil Bacteria using an Isolation Chip (iChip)

Published on: August 6, 2025

3.0K

Area of Science:

  • Microbiology
  • Biotechnology
  • Computational Biology

Background:

  • Traditional microbial isolation and cultivation methods are insufficient for discovering novel microorganisms.
  • Vast microbial diversity remains uncharacterized due to cultivation challenges.
  • Artificial intelligence (AI) offers computational solutions to advance microbial discovery.

Purpose of the Study:

  • To review the evolution of AI technologies in microbial isolation and cultivation over three decades.
  • To analyze how AI addresses key challenges in microbiology, including knowledge gaps and complex cultivation.
  • To present a five-stage framework tracking AI development in this field.

Main Methods:

  • Review of AI applications in microbial isolation and cultivation from 1997 to the present.
  • Analysis of AI's impact at genome, individual, and community levels.
  • Categorization of AI development into five distinct periods.

Main Results:

  • AI technologies have progressively enhanced microbial discovery by addressing challenges like insufficient data and complex cultivation.
  • Microbial isolation and cultivation are shifting from experience-based to data-driven and active design approaches.
  • AI integration is expanding the frontiers of microbial resource discovery and utilization.

Conclusions:

  • AI is transforming microbial resource discovery, moving beyond traditional limitations.
  • The iterative advancement of AI is crucial for unlocking the potential of microbial communities.
  • Future research should focus on further integrating AI for systematic and data-driven microbial exploration.