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Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
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Practical Real-Time Quaking-Induced Conversion for Detecting Classical Bovine Spongiform Encephalopathy and Classical

Akio Suzuki1,2, Kazuhei Sawada1, Taku Nakashima1

  • 1Laboratory of Veterinary Hygiene, Faculty of Veterinary Medicine, Graduate School of Infectious Diseases, Hokkaido University, Kita 18, Nishi 9, Kita-ku, Sapporo 060-0818, Hokkaido, Japan.

Pathogens (Basel, Switzerland)
|March 28, 2026
PubMed
Summary

Real-time quaking-induced conversion (RT-QuIC) assays can detect prions but are hindered by inhibitors. This study optimized RT-QuIC using lipid extraction and specific recombinant prion proteins (rPrPs) to improve detection of classical BSE and scrapie prions.

Keywords:
2-butanol/methanolC-BSERT-QuIClipid extractionprionscrapietransmissible spongiform encephalopathy

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