An Injectable CMCS/γ-PGA/PRP Bioadhesive With Antibacterial, Adhesive, and Regenerative Properties for Infected Wound
Wenqing Xu1,2, Jinglve Zhang1,2, Yutian Xiao1,2
1Department of Plastic and Cosmetic Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, China.
Summary
A new injectable bioadhesive (CγR) combines antibacterial, adhesive, and regenerative properties. This single-step treatment effectively heals infected wounds, offering a promising alternative to traditional wound care.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Wound Healing
Background:
- Infected traumatic wounds pose significant clinical challenges.
- Current bioadhesives lack antimicrobial and regenerative functions.
- A need exists for advanced wound management solutions.
Purpose of the Study:
- To develop an injectable bioadhesive with antibacterial, adhesive, and regenerative (AAR) capabilities.
- To evaluate the efficacy of the developed bioadhesive (CγR) in managing infected wounds.
Main Methods:
- Formulation of CγR by tuning carboxymethyl chitosan (CMCS), platelet-rich plasma (PRP), and γ-polyglutamic acid (γ-PGA).
- Assessment of bactericidal efficacy against E. coli and S. aureus.
- Measurement of wet-tissue adhesion strength.
- Evaluation of growth factor release (PDGF, TGF-β, VEGF).
- In vivo testing in a rat model of infected full-thickness wounds.
Main Results:
- CγR demonstrated >99.9% bactericidal efficacy.
- Achieved instantaneous wet-tissue adhesion (>3 kPa).
- Sustained release of key growth factors.
- In vivo: achieved sterile sealing, complete epithelialization in 6 days, 1.5-fold collagen increase, and M2 macrophage polarization.
Conclusions:
- CγR integrates antibacterial, adhesive, and regenerative properties in a single injectable formulation.
- The bioadhesive effectively manages infected wounds, promoting healing and tissue regeneration.
- CγR presents a biosafe, scalable platform for clinical translation in complex wound management.
