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Updated: Mar 30, 2026

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Directed Evolution Improves the Catalytic Efficiency of APEX2-Mediated Proximity-Dependent RNA Labeling

Gang Wang1, Yi Li1, Peiyuan Meng1

  • 1College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, Beijing National Laboratory For Molecular Sciences, PKU-IDG/McGovern Institute for Brain Research, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Beijing Advanced Center of RNA Biology (BEACON), Peking University, Beijing, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 28, 2026
PubMed
Summary

Researchers engineered ascorbate peroxidase APEX2 (APEX2) for improved proximity labeling. The enhanced APEX2 variant efficiently profiles the transcriptome near cellular structures, identifying new midbody-localized mRNA.

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