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PlLBD36 contributes to stem development through interacting with PlNF-YA1 to regulate PlEXPA15 expression in
Zhipeng Sheng1, Sihan Zhang1, Yuhan Tang1
1College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou, China.
Plant Physiology
|March 28, 2026
Summary
Researchers discovered a new pathway regulating plant stem growth. The PlNF-YA1/PlLBD36-PlEXPA15 cascade promotes stem development by enhancing cell expansion and xylem formation in herbaceous peony.
Area of Science:
- Plant Biology
- Molecular Genetics
Background:
- Plant stems are vital for growth and survival.
- Expansins (EXPs) are implicated in stem development, but upstream regulators are unknown.
Purpose of the Study:
- To elucidate the molecular mechanisms of stem development in herbaceous peony (Paeonia lactiflora).
- To identify key genes and transcription factors involved in regulating stem growth.
Main Methods:
- Yeast one-hybrid and two-hybrid screening to identify interacting proteins and DNA-binding elements.
- Gene expression analysis and protein localization studies.
- Gene silencing techniques to assess functional roles in planta.
Main Results:
- Identified PlEXPA15, an alpha-expansin highly expressed in stems, promoting cortex cell expansion and secondary xylem development.
- Discovered PlLBD36 transcription factor binds to the PlEXPA15 promoter, activating its expression and is crucial for stem development.
- Found PlNF-YA1 interacts with PlLBD36, enhancing PlEXPA15 activation and positively regulating stem development.
Conclusions:
- The PlNF-YA1/PlLBD36-PlEXPA15 transcriptional cascade is a key positive regulator of stem development in P. lactiflora.
- This pathway promotes stem growth by facilitating cortex cell expansion and secondary xylem development.
- Provides novel insights into the molecular basis of plant stem development.
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