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Related Experiment Video

Updated: Mar 30, 2026

Scanning Transmission Electron Microscopy Tomography in Virology: 3D Imaging of High-pressure Frozen, Freeze-substituted Samples

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Published on: August 6, 2025

Investigating oncolytic virus M1-Associated vesicles using 3D electron microscopy.

Lin Nie¹, Yinyin Li², Deli Song³

¹State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; Department of Electron Microscopy, Peking University First Hospital, Beijing 100034, China.

Journal of Structural Biology

|March 28, 2026

Summary

This summary is machine-generated.

Oncolytic virus M1 (OVM) assembly and release mechanisms in cancer cells were visualized using 3D electron microscopy. This study details novel cytopathic vesicles and OVM

Keywords:

Alphavirus Oncolytic Virus M1 (OVM)Three-dimensional electron microscopy cytopathic vesicles (CPVs)

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Published on: January 2, 2014

Area of Science:

Virology
Cell Biology
Oncology

Background:

Oncolytic virus M1 (OVM) shows promise for cancer therapy.
Mechanisms of OVM assembly and maturation in cancer cells require further elucidation.

Purpose of the Study:

To investigate the intracellular life cycle of OVM in cancer cells.
To characterize viral assembly, maturation, and release processes.

Main Methods:

Volume three-dimensional electron microscopy was used to examine OVM-infected Hs578T cells.

Main Results:

Four distinct cytopathic vesicles (CPVs) were identified in infected cells.
CPV-II was found to have two subtypes (CPV-II-1 and CPV-II-2), with CPV-II-2 forming intracellular networks.
Mature OVM virions were observed to be released via CPV type III.

Conclusions:

This study provides new insights into the formation of virus-associated vesicles.
The findings update the understanding of OVM's viral release pathways.
The characterization of the OVM life cycle in cancer cells is refined.