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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
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The technique...
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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Related Experiment Video

Updated: Mar 30, 2026

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA
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Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

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Single-cell RNA sequencing identifies long non-coding RNAs enriched in psoriatic epidermal subsets.

Longlong Luo1, Jan Cedric Freisenhausen2, Devin Malitha Dompage1

  • 1Dermatology and Venereology, Department of Medical Sciences, Uppsala University, Uppsala, Sweden; Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden.

Journal of Dermatological Science
|March 28, 2026
PubMed
Summary

This study maps long non-coding RNAs (lncRNAs) in psoriasis skin cells, revealing specific lncRNA patterns in keratinocytes and immune cells that may drive disease progression.

Keywords:
KeratinocyteLINC00892LINC01137Long non-coding RNAPsoriasisSingle-cell RNA sequencing

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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
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Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis

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Area of Science:

  • Dermatology
  • Genomics
  • Molecular Biology

Background:

  • Psoriasis involves skin cell dysfunction due to poor communication between keratinocytes and immune cells.
  • The role of long non-coding RNAs (lncRNAs) in this epidermal dysfunction is not well understood.

Purpose of the Study:

  • To create a single-cell atlas of lncRNA expression in healthy and psoriatic skin.
  • To identify lncRNAs specific to certain cell states and associated with psoriasis.

Main Methods:

  • Single-cell RNA sequencing (Smart-seq2) of epidermal cells (CD45+ and CD45-) from healthy and psoriatic skin.
  • Validation of key lncRNAs using RT-qPCR, RNAscope, and immunofluorescence.
  • Investigating LINC01137 regulation and function in keratinocytes.

Main Results:

  • Identified 1412 epidermal lncRNAs with cell type-specific expression.
  • Found lncRNAs selectively expressed in psoriasis-associated cell states.
  • LINC01137, enriched in activated keratinocytes, influences TGF-β signaling; LINC00892 is found in lesional T cells.

Conclusions:

  • This research maps the single-cell lncRNA landscape of psoriatic epidermis.
  • Distinct lncRNA signatures in keratinocytes and immune cells suggest their role in psoriasis pathogenesis.