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Related Concept Videos

Attenuated Total Reflectance (ATR) Infrared Spectroscopy: Overview01:13

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Related Experiment Video

Updated: Mar 30, 2026

Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS
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Biomolecular Detection employing the Interferometric Reflectance Imaging Sensor IRIS

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Multi-parametric interferometric reflectance imaging sensor.

Mete Aslan1, Stephen Snekvik1, Elif Seymour2

  • 1Department of Electrical and Computer Engineering, Boston University, 8 St. Mary's St., Boston, MA, 02125, USA.

Scientific Reports
|March 28, 2026
PubMed
Summary
This summary is machine-generated.

We developed a dual-wavelength sensor for real-time, label-free binding kinetics. This multi-parametric Interferometric Reflectance Imaging Sensor (MP-IRIS) minimizes bulk effects for accurate multiplexed measurements.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Surface Science

Background:

  • Label-free biosensing is crucial for real-time molecular interaction analysis.
  • Existing methods often suffer from bulk effects and require complex experimental setups.
  • Accurate kinetic measurements are essential for drug discovery and diagnostics.

Purpose of the Study:

  • To present a novel dual-wavelength, multi-parametric Interferometric Reflectance Imaging Sensor (MP-IRIS).
  • To achieve real-time, label-free, and multiplexed binding kinetic measurements.
  • To eliminate or significantly reduce the impact of bulk effects in biosensing.

Main Methods:

  • Utilized a dual-wavelength Interferometric Reflectance Imaging Sensor (MP-IRIS) platform.
  • Implemented real-time dynamic tracking and correction of solution conditions to mitigate bulk effects.
  • Employed multi-wavelength data acquisition to enhance measurement repeatability and reduce quantification errors.
  • Performed proof-of-concept DNA hybridization assays for validation.

Main Results:

  • Demonstrated a significant reduction in bulk effect to 3 pg/mm² with a bulk refractive index change of [Formula: see text] RIU.
  • Achieved improved repeatability and reduced quantification error through multi-wavelength data acquisition.
  • Validated accurate, multiplexed binding measurements under diverse chip conditions using DNA hybridization assays.
  • Maintained high sensitivity comparable to previous Interferometric Reflectance Imaging Sensor (IRIS) designs.

Conclusions:

  • The MP-IRIS platform enables accurate, real-time, label-free kinetic measurements with minimal bulk effect.
  • Multi-wavelength data acquisition enhances robustness against variations in chip manufacturing and surface treatment.
  • The system's reduced complexity and high sensitivity offer broad potential applications, including small-molecule analysis.