Protocol for characterization of spatiotemporal network dynamics in cortical and hippocampal assembloids
Asia R Guzman1, Colin M McCrimmon1, Daniel Toker1
1Department of Neurology, University of California, Los Angeles, David Geffen School of Medicine, Los Angeles, CA 90095, USA.
Abstract:
This protocol generates neural assembloids from human induced pluripotent stem cells (hiPSCs) to model human brain circuitry. It details the differentiation of excitatory-predominant hippocampal (Hc) and cortical (Cx) organoids and their fusion with inhibitory interneuron-predominant ganglionic eminence (GE) organoids. We then detail procedures for maintaining assembloids to promote interneuron migration and network integration, followed by functional assessment using two-photon calcium imaging to measure neuronal activity. For complete details on the use and execution of this protocol, please refer to McCrimmon et al.1.
