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Published on: November 2, 2020
Hsa-miR-514a-3p suppresses oral squamous cell carcinoma(OSCC) progression by targeting COL1A1
Shensui Li1, Xudong Tian2, Jukun Song2
1School of Clinical Medicine, Guizhou Medical University, Guiyang, 550001, Guizhou Province, China; Department of Oral and Maxillofacial Surgery, Guizhou Medical University, Guiyang, 550004, Guizhou Province, China.
Objective(S):
The research aimed to investigate the impact of hsa-miR-514a-3p on the proliferation and invasion of oral squamous cell carcinoma (OSCC), and to identify its potential target gene COL1A1.
Design:
The expression patterns, functional enrichment (Gene Set Enrichment Analysis, GSEA), and copy number variations (CNV) of miR-514a-3p and COL1A1 were analyzed using public datasets (E_MTAB_8588, GSE75538, TCGA-HNSC, and Human Protein Atlas, HPA). The expressions of miR-514a-3p and COL1A1 in clinical samples were detected by immunohistochemistry (IHC) and RT-qPCR. Lentiviral vectors (oe-miR-514a-3p, sh-miR-514a-3p, oe-COL1A1, and sh-COL1A1) were transfected into SCC-25 cells. The expressions of COL1A1, epithelial-mesenchymal transition (EMT)-related molecules, and matrix metalloproteinases (MMPs) were detected by RT-qPCR, Western blotting, and ELISA. Cell functions were evaluated using colony formation assay, Transwell assay, flow cytometry, and CCK-8 assay. A dual-luciferase reporter assay was used to verify the binding between miR-514a-3p and COL1A1.
Results:
Hsa-miR-514a-3p showed a significant downregulation in OSCC tissues compared to adjacent controls. Hsa-miR-514a-3p overexpression led to reduced OSCC cell proliferation and invasion, whereas its downregulation resulted in increased these processes. hsa-miR-514a-3p directly targeted COL1A1, with negative correlation in OSCC samples. Silencing COL1A1 mimicked the suppressive effects of hsa-miR-514a-3p, and COL1A1 overexpression partially reversed them.
Conclusion:
This study demonstrates that hsa-miR-514a-3p inhibits malignant phenotypes of OSCC cells by targeting COL1A1, providing insights into potential therapeutic targets for OSCC management.
Insights
Hsa-miR-514a-3p inhibits oral squamous cell carcinoma (OSCC) progression by targeting COL1A1. Downregulation of this microRNA promotes OSCC cell proliferation and invasion, highlighting its therapeutic potential.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Oral squamous cell carcinoma (OSCC) is a prevalent malignancy with complex molecular underpinnings.
- MicroRNAs (miRNAs) play crucial roles in cancer development and progression.
- Identifying key regulatory pathways is essential for developing novel therapeutic strategies for OSCC.
Purpose of the Study:
- To investigate the role of hsa-miR-514a-3p in OSCC proliferation and invasion.
- To identify COL1A1 as a potential target gene of hsa-miR-514a-3p.
- To elucidate the molecular mechanism by which hsa-miR-514a-3p influences OSCC malignancy.
Main Methods:
- Analysis of public datasets (e.g., TCGA-HNSC) and clinical samples for expression patterns of hsa-miR-514a-3p and COL1A1.
- Functional assays including cell proliferation (CCK-8, colony formation), invasion (Transwell), and apoptosis (flow cytometry) in OSCC cells with manipulated miR-514a-3p and COL1A1 levels.
- Validation of the direct interaction between hsa-miR-514a-3p and COL1A1 using dual-luciferase reporter assays.
Main Results:
- Hsa-miR-514a-3p was significantly downregulated in OSCC tissues.
- Overexpression of hsa-miR-514a-3p suppressed OSCC cell proliferation and invasion, while its inhibition promoted these processes.
- Hsa-miR-514a-3p directly targets COL1A1, and their expression levels showed a negative correlation in OSCC samples.
Conclusions:
- Hsa-miR-514a-3p acts as a tumor suppressor in OSCC by targeting COL1A1.
- The hsa-miR-514a-3p/COL1A1 axis is a critical regulator of OSCC cell malignancy.
- This finding offers potential therapeutic targets for managing OSCC.
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