Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

12.1K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
12.1K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

3.6K
3.6K
Mutations01:39

Mutations

96.4K
Overview
96.4K
Mutations01:35

Mutations

45.5K
Mutations are changes in the sequence of DNA. These changes can occur spontaneously or they can be induced by exposure to environmental factors. Mutations can be characterized in a number of different ways: whether and how they alter the amino acid sequence of the protein, whether they occur over a small or large area of DNA, and whether they occur in somatic cells or germline cells.
Chromosomal Alterations Are Large-Scale Mutations
While point mutations are changes in a single nucleotide in...
45.5K
Mutations01:39

Mutations

13.7K
13.7K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

9.2K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Maternal obesity induces activator protein 1-mediated inflammatory response to impair embryonic neurogenesis.

The Journal of physiology·2026
Same author

Exercise attenuates polycystic ovary syndrome development via improved mitochondrial proteostasis.

American journal of physiology. Endocrinology and metabolism·2026
Same author

Expansion of low-price cigarette market and its implications for cigarette tax revenue: Evidence from Bangladesh.

Tobacco induced diseases·2025
Same author

RelA Inhibits Embryonic Myogenesis by Coordinately Regulating a Novel Distal Enhancer of Myogenin.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Leaky waveguide biosensors for label-free measurement of human serum albumin.

The Analyst·2025
Same author

Balancing LncRNA H19 and miR-675 Bioconversion as a Key Regulator of Embryonic Myogenesis Under Maternal Obesity.

Journal of cachexia, sarcopenia and muscle·2025

Related Experiment Video

Updated: Mar 31, 2026

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
06:18

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems

Published on: April 26, 2019

6.5K

Nonsense mutations can increase mRNA levels.

Precious O Owuamalam1, Md Nazmul Hossain1, Saverio Brogna1

  • 1School of Biosciences and Birmingham Centre for Genome Biology (BCGB), University of Birmingham, Edgbaston B15 2TT, UK.

Biology Open
|March 30, 2026
PubMed
Summary

Nonsense mutations can unexpectedly increase mRNA levels, challenging current models of nonsense-mediated decay (NMD). This study reveals positional context significantly impacts mRNA abundance following premature translation termination.

Keywords:
GFPNMDSplicingUPF1mRNA surveillance

More Related Videos

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
12:21

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Published on: December 13, 2014

13.2K
Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
09:04

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

8.3K

Related Experiment Videos

Last Updated: Mar 31, 2026

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems
06:18

Measurement of Specific Mycobacterial Mistranslation Rates with Gain-of-function Reporter Systems

Published on: April 26, 2019

6.5K
Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
12:21

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Published on: December 13, 2014

13.2K
Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis
09:04

Studying Ribonucleotide Incorporation: Strand-specific Detection of Ribonucleotides in the Yeast Genome and Measuring Ribonucleotide-induced Mutagenesis

Published on: July 26, 2018

8.3K

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Expression Regulation

Background:

  • Nonsense mutations often lead to reduced mRNA levels due to nonsense-mediated mRNA decay (NMD).
  • The impact of premature translation termination codon (PTC) position on mRNA abundance is not fully understood.
  • NMD is a crucial cellular surveillance mechanism regulating mRNA quality.

Purpose of the Study:

  • To investigate how the location of PTCs within a gene influences mRNA levels.
  • To explore the relationship between PTC position, NMD, and transcript stability.
  • To determine if splicing affects NMD efficiency and PTC outcomes.

Main Methods:

  • Introduction of PTCs at 15 distinct positions in a GFP reporter gene in Schizosaccharomyces pombe.
  • Quantification of mRNA levels and transcript stability for various PTC constructs.
  • Analysis of NMD pathway involvement through UPF1 deletion.
  • Examination of spliced and unspliced transcript variants.

Main Results:

  • PTCs in the first third of the gene consistently reduced mRNA levels.
  • Most downstream PTCs resulted in minimal reduction or even increased mRNA levels.
  • Increased mRNA abundance was not due to reduced transcript turnover.
  • UPF1 deletion modulated mRNA levels, affecting both reduced and increased transcripts.
  • Splicing generally reduced mRNA levels for downstream PTCs, but sometimes suppressed reduction for early PTCs.

Conclusions:

  • The position of a nonsense mutation significantly impacts mRNA abundance, with downstream PTCs potentially increasing levels.
  • These findings expand upon current NMD models and highlight context-dependent regulation of mRNA decay.
  • Understanding these effects is crucial for interpreting nonsense mutation consequences and designing gene expression systems.